Black Lingzhi, Amauroderma subresinosumof the the family Ganoderma, contains numerous bioactive compounds, such as sterols, flavonoids, phenols, fatty acids, amino acids, polysaccharides, and triterpenes. Whileactive constituents, distribution, and morphological characteristics of black lingzhi have been studied in Vietnam, there has been limited research conducted to determine the ideal culture conditions that promote mycelial growth. This study aimed to determine the optimal culture conditions for mycelialgrowthof Amauroderma subresinosumstrain Am-4, collected from Bao Lam, Lam Dong province. Strain Am-4 was cultured on Ohta, PDA, SDA, MEA, MCM, and Czapek media under different pH levels, temperatures, carbon, nitrogen, and mineral salt sources. Our results indicated that strain Am-4 exhibited rapid mycelialgrowth on MEA medium at 25°Cand pH5. The strain showed the highest growth rate when cultured in a medium containing glucose and NaNO3as the carbon and nitrogen sources, respectively, with the optimal glucose/NaNO3ratio being 10:1. Additionally, ZnSO4.7H2O was found tobetheoptimal mineral salt source. Under the optimized culture conditions, strain Am-4 exhibited fast mycelial growth with a compact density.