Received: 03-03-2021 / Accepted: 15-07-2021
The study aimed to select Bacillus subtilisstrains which are capable of producing β-glucosidase to hydrolyse ginsenosidefrom the root of Panax pseudoginsenginto the bioactive compound Rg3 and CK. Twenty four strains were primary screened for the β-glucosidase n using 0.1% azo-CMC and 0.3% azo-Avicel. The classification of strains based on the morphology and phylogeny was analysed by the sequence of 16S. β-glucosidase activity obtained from strains was determined by DNS method using PNP-β-D-glucopyranoside substrate. The hydrolysis of ginsenoside to Rg3 with enzyme β-glucosidase was identified using thin layer chromatography (TLC). Rg3 and CK content were determined using high performance liquid chromatography HPLC). Results showed that Bacillus subtilisstrain NL812 produced β-glucosidase with the highest activity. The β-glucosidase released from Bacillus subtilisstrain NL812 hydrolyzed ginsenoside from Panax pseudoginsenginto Rg3 and CK after 30 min. with Rg3 content increased by 50.3% andCK content increased by 47.7%.