Received: 18-04-2017 / Accepted: 26-07-2017
In mammals, reproduction is the ability to generate next generation through the fertilization of a mature egg and a sperm. Nowadays, with the development of modern reproductive biotechnology, several advanced techniques have been applied and able to generate new generations from somatic cellsandstem cells or from immature eggs and sperms. In the present paper, the advances in reproduction over the past 20 yearsare sumarized and presented including: (1) intracytoplasmic sperm injection (ICSI) and application of this method to treat infertility and production of transgenic animals, (2) round spermatid injection (ROSI) and itsapplication on treatment of infertility without spermatozoa, (3) Somatic cell nuclear transfer (SCNT), also known as animal cloning, and (4) the method to generate new generation by pluripotent stem cells injected into tetraploid blastocysts.The application potential of the modern reproductive methods in medicine and agriculturewas discussed.
