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• Study on Preparation of Chitosan Nanocapsules Transduced with Trans- cinnamaldehyde for Communication Inhibition in E. coli Top10

  Dong Huy Gioi, Nguyen Thanh Hao, Nguyen Thi Bich Luu

  Received: 27-02-2023 / Accepted: 05-01-2024

   Abstract PDF (VIETNAMESE)

  This study was conducted with the aim of preparing chitosan nanocapsules transduced with trans-cinnamaldehyde for communication inhibition in bacteria. We used emulsification method to synthesize nanocapsule and nanoemulsion complexes. The nanocapsule and nanoemulsion complexes were transduced with trans-cinnamaldehyde using an oil nucleus and chitosan as cover. The nanocomplex had a uniform particle structure, low dispersion, and the size ranges from 120 to 150nm. Both nanocapsule and nanoemulsion were stable in bacterial culture after 24h of incubation. The trans-cinnamaldehyde transduction efficiency for the nanocapsule and nanoemulsion complexes was 86 and 73%, respectively. Both complexes released the drug through two stages, slowly in the first 6 hours and followed by continuous drug release after 12 hours, reaching 35 and 45%, respectively, for the nanocapsule and nanoemulsion. Bioassays with the E. coli Top 10 biosensor showed that nanocapsule was superior to nanoemulsions in inhibiting bacterial communication, also known as quorum sensing. In the best formulation, nanocapsule inhibited the communication process in this bacterium up to 65%.

• Evaluation of Quorum Sensing Communication between Pathogenic Bactria Causing Acute Hepatopancreatic Necrosis Disease and Vibrio Alginolyticus

  Tran Thi Thuy Ha, Nguyen Thi Hanh, Pham The Viet, Phan Thi Van, Truong Thi My Hanh

  Received: 01-08-2018 / Accepted: 26-08-2018

   Abstract PDF (VIETNAMESE)

  Bacterial cell-to-cell communication(quorum sensing - QS) is associated with the virulence of pathogenic bacteria. The study was conducted to evaluatethe QS communication between Vibrioparahaemolyticus - pathogenic agent causing acute hepatopancreatic necrosis disease (AHPND) and V. alginolyticus- non-AHPND and, concurrently, to determine the role of cinnamon essential oil for QS between two bacterial species mentioned above. The methods used included traditional bacterial culture and PCR technique. Results showed that V. alginolyticus received virulence gene AHPND from V. parahaemolyticusunder the culture condition with three times of heat shock at 400C (5 min) and 700C (2 min) via QS mechanism. In addition, cinnamon essential oil with a dose of 0.1μL/600μL disrupted the QS communication system of bacteria, causing V. alginolyticusimpossible to receive virulence gene causing AHPND from V. parahaemolyticus.