Received: 20-12-2015 / Accepted: 22-05-2017
In the present study, we propagatedHibicus sagittifoliusKurz plants usingstem nodalculture. After 30 days of culture, the results showed that MS medium supplemented with 0.3 mg/l BA, 0,1 mg/l NAA, 30 g/l sucrose, 8 g/l agar, pH 5.8 was the best medium for shoot regeneration and growth (5.00 shoots at the height of 3.65 cm). First stem nodes to fourth stem nodes were suitable materials for in vitroculture of Hibicus sagittifoliusKurz (3.00 - 6.90 shoot/explant). In the same culture medium, all samples did not regenerate the root in medium without activated charcoal, but when supplemented with activated charcoal, 100% samples regenerated root and shoot growth.MS medium containing 0.1 - 1 mg/l IBA, 30 g/l sucrose, 8 g/l agar, pH 5.8 was suitable for root regeneration. Coconut fiber powder was suitable substrate for the transfer of plantlets to greenhouse.Hibicus sagittifoliusKurz plantlets flowered after 80 days cultivation when grown on coconut fiber powdersubstrate.