Received: 07-07-2014 / Accepted: 13-08-2014
The research was performedto investigate the in vitroantibacterial effect of Clerodendrum fragrans vent. (roots,branchesand leaves) extract on 2 pathogenic bacteria (E. coliandSalmonella spp.) isolated from feces of diarrhea-infectedpiglets. The experimental clinical treatment of the extracts was also performed on diarrhea piglets. The results showed that ethanol 70% branch extract possessed the best in vitro antibacterial effect on both of tested bacteria with the largest diameter of inhibitory zone (E. coli- 23 mm and Salmonella spp. - 25 mm), while the root extract had only small inhibition zone and leaf extract had no inhibition zone. We compared the efficacy of Clerodendrum fragrans vent. branch by different extract solvents, including ethanol 35%, ethanol 70%, axit acetic 5% and aceton 70%. All of these branch extracts showed in vitro inhibition effect with E.coliand Salmonella spp., in which ethanol 35% extract possessed the best effect, shown by the largest diameter of inhibitory zone (E. coli-30,67 mm; Salmonella spp. - 32,33 mm). Ethanol 35% extract also had good in vitroantibacterial effect with large inhibitory zone (>20mm) on E. coli with antibacterial resistant palsmid (kanamycin resistantE. coliTop 10 pPS1; ampicillin resistantE. coliTop 10 pJET 1.2/blunt). The experimantal clinical treatment of ethanol 35% Clerodendrum fragrans vent. branch extract on diarrhea piglets also showed good results.
