Received: 26-08-2013 / Accepted: 15-11-2013
Studies of micropropagation of D. nobileLindl. were conducted in order to conserve and increase the genetic pool of this precious orchid species. The results showed that the suitable materials used for micropropagation were 5 month old seed capsules. The most suitable medium for germination and protocorm formation was MS+100ml coconut juice+10g saccharose+6.0g agar/liter. For in vitro propagation by traditional method, the optimal medium for propagation of protocorms was KC+100ml coconut juice+10g saccharose+6.0g agar/liter. The most appropriate medium for rapid in vitropropagation of shoots was MS+100ml coconut juice+10g saccharose+6.0g agar/liter. For in vitro propagation by improved method using liquid medium and cotton buttons combined with shaking or using liquid medium and ventilative buttons (cellulose acetate) combined with shaking had increased multiplication rate of protocorms (1.9 and 2.3 fold in comparing with the control). The propagation using solid medium with ventilation buttons reduced 25% of saccharose added to medium, and increased the multipliecation rate of protocorms up to 1.4 fold in comparison with propagation by the classical method. Similarly, rapid in vitro propagation using bioreactor redued the time of propagation by half. The most optimal medium used regenerating intact plants of D. nobilewas RE+10g saccharose +0.5g activated carbon+6.0g agar/liter combined with light intensity of 2300lux.
