Isolation and Identification of Soft Rot Bacteria on Dendrobium AnosmumOrchid and Evaluation of Inhibitory Effects of Some Actinomycetes

Received: 20-07-2021

Accepted: 09-12-2021

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Issue: Vol. 20 No. 1 (2022)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

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Hien, P., Truong, D., Hoan, D., Dao, T., Thu, N., & Canh, N. (2024). Isolation and Identification of Soft Rot Bacteria on Dendrobium AnosmumOrchid and Evaluation of Inhibitory Effects of Some Actinomycetes. Vietnam Journal of Agricultural Sciences, 20(1), 74–81. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/934

Isolation and Identification of Soft Rot Bacteria on Dendrobium AnosmumOrchid and Evaluation of Inhibitory Effects of Some Actinomycetes

Pham Hong Hien (*) 1 , Diem Dang Truong 2 , Duong Van Hoan 2 , Tran Thi Dao 2 , Nguyen Thi Thu 2 , Nguyen Xuan Canh 2

  • 1 Ban Khoa học và Hợp tác quốc tế, Viện Khoa học Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Soft rot, Dendrobium anosmum, Bacillus pumilus, Bacillus altitudini, Streptomyces

    Abstract


    Bacterial soft rot is one of the most common diseases and mainly affects plants including orchid Dendrobium anosmum. The bacteria causing soft rot penetrates plant tissue, causing soft rot through the wound. This study aimed to íolate and identify bacterial strains capable of causing soft rot disease on orchids and select actinomycetes capable of antagonizing pathogenic bacterial strains. From five samples of orchid leaves showing symptoms of soft rot disease collected in Bac Giang, two strains of bacteria capable of causing soft rot disease were-isolated and the pathogenicity determined by artificial re-infection method. Bacterial strains were identified as Bacillus pumilusVK3 and B. altitudinisVK5B. By usingthe disk-diffusion method, from six Streptomycesstrains, XK7 Streptomycesstrain was selected for its antagonistic effect against B. pumilusVK3 and B. altitudinisVK5B-with the diameters of clear zone of17 ± 0,07mm and 14 ± 0,05mm, respectively.

    References

    Alic S., Naglic T., Znidaric M.T., Peterka M., Ravnikar M. & Dreo T. (2017). Putative new species of the genus Dickeyaas major soft rot pathogens in Phalaenopsisorchid production. Plant Pathology. 66(8): 1357-1368.

    Babana A.H., Bathily H., Samaké F., Maïga K., Traoré D. & Dicko A. (2011). Microbiological control of bacterial soft rot caused by Bacillus pumilusOD23 on potato. British Microbiology Research Journal. 1(3): 41-48.

    Balouiri M., Sadiki M. & Ibnsouda S.K. (2015). Methods for in vitroevaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis. 6(2): 71-79.

    Bathily H., Babana A.H. & Samaké F. (2010). Bacillus pumilus, a new pathogen on potato tubers in storage in Mali. African Journal of Microbiology Research. 4(20): 2067-2071.

    Cating R.A., Hoy M.A., & Palmateer A.J. (2012). A comparison of standard and high-fidelity PCR: evaluating quantification and detection of pathogen DNA in the presence of orchid host tissue. Plant Disease. 96(4): 480-485.

    Cating R.A. & Palmateer A.J. (2011) Bacterial soft rot of Oncidiumorchids caused by a Dickeyasp. (Pectobacterium chrysanthemi) in Florida. Plant Dis. 95(1): 74-74.

    Chang Y.Y., Chu Y.W., Chen C.W., Leu W.M., Hsu H.F. & Yang C.H. (2011). Characterization of oncidium‘gower ramsey’ transcriptomes using 454 GS-FLX pyrosequencing and their application to the identification of genes associated with flowering time. Plant and Cell Physiology. 52(9): 1532-1545.

    Đào Thanh Vân & Đặng Thị Tố Nga (2008). Giáo trình Hoa lan. Truy cập từ http://tailieudientu.lrc.tnu. edu.vn/Upload/Collection/brief/brief_1308_10008_Small_09.Giaotrinhhoalan.pdf ngày 24/08/2021.

    Elbanna K., Elnaggar S. & Bakeer A. (2014). Characterization of Bacillus altitudinisas a new causative agent of bacterial soft rot. Journal of Phytopathology.162(11-12): 712-722.

    Eum S.M., Gale S., Yukawa T. & Lee N.S. (2011). Phylogenetic and conservation status of the endangered terrestrial orchid Nervilia nipponica(Orchidaceae) in Korea. Biochem Syst Ecol. 39(4-6): 635-642.

    Joko T., Kiswanti D., Hanudin & Subandiyah S. (2011). Occurence of bacterial soft-rot of Phalaenopsis orchids in Yogyakarta and West Java, Indonesia. In Proceeding of Internasional Seminar on Natural Resources, Climate Change dan-Food Security in Developing Countries. pp. 27-28.

    Joo G.J. (2005). Production of an anti-fungal substance for biological control of Phytophthora capsicicausing Phytophthora blight in red peppers Streptomyceshalstedii. Biotechnology Letter. 27(3): 201-205.

    Keith L.M., Sewake K.T. & Zee F.T. (2005). Isolation and characterization of Burkholderia gladioli from orchids in Hawaii. Plant Dis. 89: 1273-1278.

    Lemjiber N., Naamani K., Merieau A., Dihazi A., Zhar N., Jediyi H. & Boukerb A.M. (2021). Identification and Genomic Characterization of Pathogenic Bacillus altitudinisfrom Common Pear Trees in Morocco. Agronomy. 11(7): 1344.

    Liau C.H., Lu J.C., Prasad V., Hsiao H.H., You S.J., Lee J.T., Yang N.S., Huang H.E., Feng T.Y., Chen W.H. & Chan M.T. (2003). The sweet pepper ferredoxin-like protein (pflp) conferred resistance against soft rot disease in Oncidiumorchid. Transgenic Research. 12(3): 329-336.

    Lin Y.H., Lee P.J., Shie W.T., Chern L.L. & Chao Y.C. (2015). Pectobacterium chrysanthemias the dominant causal agent of bacterial soft rot in Oncidium“Grower Ramsey”. European Journal of Plant Pathology. 142(2); 331-343.

    Masoomi A.F., Jabbari L., Khayam N.R. & Aalami A. (2016). A simple and rapid system for DNA and RNA isolation from diverse plants using handmade kit. Protocol Exchange.

    Nuryani W., Hanudin H., Yusuf E.S. & Budiarto K. (2018). Physico-Chemical, Viability Evaluations and Efficacy Assessment of Bacillus subtilisAgainst Soft Rot Disease in Phalaenopsis. AGRIVITA, Journal of Agricultural Science. 40(3): 425-433.

    Palma M.A., Chen Y.J., Hall C., Bessler D. & Leatham D. (2010). Consumer preferences for potted orchids in the Hawaiian market.Hortechnology. 20(1): 239-244.

    Peng Q., Yuan Y. & Gao M. (2013). Bacillus pumilus, a novel ginger rhizome rot pathogen in china. Plant Disease. 97(10): 1308-1315.

    Pulawska J., Mikicinski A. & Orlikowski L.B. (2013) Acidovorax cattleyae the causal agent of bacterial brown spot of Phalaenopsislueddemanniana in Poland. Journal of Plant Pathology. pp. 407-410.

    Shimizu M., Yazawa S. & Ushijima Y. (2008). A promising strain of endophytic Streptomycessp. for biological control of cucumber anthraxcnose. Journal of General Plant Pathology. 75(1): 27-36.

    Simone G.W. & Burnett H.C. (2002). Diseases caused by bacteria and fungi. Orchid pests and diseases. American Orchid Society, Delray Beach. pp. 50-73.

    Sudarsono S., Elina J., Giyanto & Sukma D. (2018). Pathogen Causing PhalaenopsisSoft Rot Disease -16S rDNA and Virulence Characterisation. Plant Protection Science. 54(1): 1-8.

    Vũ Thị Hoàn, Phan Quốc Hưng & Vũ Thị Xuân Hương (2020). Phân lập, tuyển chọn vi khuẩn đối kháng với vi khuẩn gây bệnh thối mềm trên cây lan Hồ điệp. Tạp chí Nông nghiệp và Phát triển nông thôn. 3 + 4: 5-12.