Effectsof CarbonandNitrogenSources and Mineral Salts on the Mycelium Growth of Snow Mushroom Tremella fuciformisStrain Tre-1

Received: 09-06-2021

Accepted: 30-08-2021

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Issue: Vol. 19 No. 11 (2021)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Giang, N., Thao, N., Ha, T., & Trinh, N. (2024). Effectsof CarbonandNitrogenSources and Mineral Salts on the Mycelium Growth of Snow Mushroom Tremella fuciformisStrain Tre-1. Vietnam Journal of Agricultural Sciences, 19(11), 1522–1530. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/911

Effectsof CarbonandNitrogenSources and Mineral Salts on the Mycelium Growth of Snow Mushroom Tremella fuciformisStrain Tre-1

Nguyen Van Giang (*) 1 , Nguyen Thi Phuong Thao 2 , Tran Thu Ha 3 , Nguyen Duy Trinh 4

  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 2 Học viện Nông nghiệp Việt Nam
  • 3 Trung tâm Nghiên cứu và Phát triển Nấm, Viện Di truyền Nông nghiệp
  • 4 Trung tâm NC và PT nấm, Viện Di truyền Nông nghiệp

    • Keywords

    Carbon and nitrogen sources, mineral salts, mycelium growth, snow mushroom(Tremella fuciformisI)

    Abstract


    Snow mushroom (Tremella fuciformis) is a high-value edible mushroom. Polysaccharide compounds from T. fuciformisexhibited various biological activities such as improving the immune system and antioxidant, anti-tumor, and anti-inflammatory activity. This study was carried out to investigate the effects of different carbon sources (glucose, fructose, lactose, maltose, sucrose) and nitrogen sources (yeast extract, peptone, NH4NO3, (NH4)2SO4, (NH4)2HPO4and NH4Cl) and mineral salts (MgSO4.7H2O, KH2PO4and ZnSO4) on the mycelium growth of the snow mushroom strain Tre-1. Snow mushroom Tre-1 was grown on PA medium supplemented with 20g/l of different carbon sources, including glucose, lactose, maltose, fructose, sucrose, to evaluate the effect of carbon source on the growth and development of mycelium. Similarly, to investigate the effect of nitrogen source on snow mushroom mycelia, experiments were carried out on PA medium supplemented with 20 g/l glucose and 1.0 g/l different nitrogen sources, including yeast extract, peptone, NH4NO3, (NH4)2SO4, (NH4)2HPO4and NH4Cl. The effect of glucose/peptone ratio and a mineral source on the growth of mycelia of Tre-1 mushroom was evaluated based on the experimental results of growing Tre-1 mycelia on PA medium containing 1 g/l of peptone added 15, 20, 25, 30, 35 and 40 g/l glucose respectively, and 0.25 g/l mineral salts MgSO4.7H2O, KH2PO4and ZnSO4. Results indicated that the optimal carbon and nitrogen sources for the mycelial growth of T. fuciformisstrain Tre-1 were glucose and peptone, respectively. The optimum glucose/peptone ratio was in the range of 20:1 to 25:1. MgSO4.7H2O was identified as the most effective source.

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