Effects of Plant Growth Regulators on in vitroPropagation of Kaempferia galanga L.

Received: 08-03-2021

Accepted: 16-06-2021

DOI:

Views

6

Downloads

1

Issue: Vol. 19 No. 8 (2021)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Son, D., Thuc, C., Hai, T., Hien, P., Hang, P., Hai, N., … Tam, D. (2024). Effects of Plant Growth Regulators on in vitroPropagation of Kaempferia galanga L. Vietnam Journal of Agricultural Sciences, 19(8), 1084–1090. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/865

Effects of Plant Growth Regulators on in vitroPropagation of Kaempferia galanga L.

Dinh Truong Son (*) 1 , Chu Dinh Thuc 1 , Tran Van Hai 1 , Pham Hong Hien 2 , Pham Thi Thu Hang 1 , Nguyen Thanh Hai 1 , Nguyen Thi Lam Hai 1 , Dang Thi Thanh Tam 3

  • 1 Khoa Công nghệ sinh học, Họcviện Nông nghiệp Việt Nam
  • 2 Viện Khoa học Nông nghiệp Việt Nam
  • 3 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Kaempferia galanga L., in vitropropagation, medicinal plant

    Abstract


    The study aims to develop a simple protocol for in vitro regeneration of Kaempferia galanga L., a local medicinal plant in Vietnam. The rhizome buds (2-3cm) were collected as initial explants and surface sterilized with 0.1% HgCl2for 10m. Initial in vitro shoots were cultured in MS medium supplement with different plant regulatorsincluding BA, Kinetin, NAA and IAA. The combination of 2 mg/l BA and 0.5 mg/l NAA induced the highest shoot multiplication with a mean of 5.03 shoots per explantafter 4 weeks of culture. In this optimal shoot induction medium, in vitro shoots also induced vigorousshoots and roots after five cycles of multiplication. The proposed medium is an efficient medium for in vitro rapid propagation or conservation of K. galanga L. 

    References

    Chithra M., Martin K.P., Sunandakumari C. & Madhusoodanan P.V. (2005). Protocol for rapid propagation, and to overcome delayed rhizome formation in field established in vitroderived plantlets of Kaempferia galangaL. Scientia Horticulturae.104(1): 113-120.

    Đỗ Huy Bích, Đặng Quang Chung, Bùi Xuân Chương, Nguyễn Thượng Dong, Đỗ Trung Đàm, Phạm Văn Hiển, Vũ Ngọc Lộ, Phạm Kim Mãn & Đoàn Thị Nhu (2004). Cây thuốc và động vật làm thuốc ở Việt Nam (1). Nhà xuất bản Khoa học và kỹ thuật Hà Nội. tr. 782-785.

    Kumar A. (2020). Phytochemistry, pharmacological activities and uses of traditional medicinal plant Kaempferia galangaL. – An overview. Journal of Ethnopharmacology.253: 112667.

    Murashige T. & Skoog F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum.15(3): 473-497.

    Parida R., Mohanty S., Kuanar D. & Nayak S. (2010). Rapid multiplication and in vitro production of leaf biomass in Kaempferia galangathrough tissue culture. Electronic Journal of Biotechnology. 13.

    Rahman M.M., Amin M.N., Ahamed T., Ahmad S., Habib A., Ahmed R., Ahmed M.B. & Ali M.R. (2005).In vitrorapid propagation of black thorn (Kaempferia galangaL.): A rare medicinal and aromatic plant of Bangladesh. Journal of Biological Sciences.5: 300-304.

    Shetu H., Trisha K., Sikta S., Anwar R., Rashed S.S., Rashed B. & Dash P. (2018). Pharmacological importance of Kaempferia galanga(Zingiberaceae): A mini review. International Journal of Pharmacy and Pharmaceutical Sciences.3: 32-39.

    Shirin F., Kumar S. & Mishra Y. (2000). In vitroplantlet production system for Kaempferia galanga, a rare Indian medicinal herb. Plant Cell, Tissue and Organ Culture.63(3): 193-197.

    Yao F., Huang Y., Wang Y. & He X. (2018). Anti-inflammatory diarylheptanoids and phenolics from the rhizomes of kencur (Kaempferia galangaL.). Industrial Crops and Products.125: 454-461.