Application of PCR Techniques for Diagnosis of Feline Herpesvirus -1 (FHV-1) in Cats

Received: 01-12-2020

Accepted: 26-04-2021

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Issue: Vol. 19 No. 5 (2021)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Ngoc, N., Phan, L., Hanh, N., Luu, H., Ngoc, T., Yen, N., & Hung, L. (2024). Application of PCR Techniques for Diagnosis of Feline Herpesvirus -1 (FHV-1) in Cats. Vietnam Journal of Agricultural Sciences, 19(5), 616–624. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/822

Application of PCR Techniques for Diagnosis of Feline Herpesvirus -1 (FHV-1) in Cats

Nguyen Thi Ngoc (*) 1 , Le Van Phan 1 , Ngo Thi Hanh 1 , Ho Chi Luu 1 , Trinh Thi Bich Ngoc 1 , Nguyen Thi Yen 1 , Le Van Hung 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Cats, Feline Herpesvirus-1, FHV-1, PCR

    Abstract


    Feline Herpesvirus 1 (FHV-1), an enveloped double-stranded DNA virus, is one of the major pathogens of feline upper respiratory tract disease (URTD) and ocular disease. This study aimedto applyPCR techniques for diagnosis ofFHV-1. The study was conducted on 317 cats. The results showed that there were 62 out of 317 cats (19.56%)identified with the signs of FHV-1 infected cats by the clinical diagnosis method. Twenty-two out of 62 cats were positive with FHV-1 by PCR method accounting for 35.48%.Twenty-two out of 317 cats were positive with FHV-1 accounting for 6.94%compared with a total of the investigated cats. The Feline Herpesvirus -1 infection rate in cats < 12 months higher than adult cats and FHV-1 infection rate in unvaccinated cats higher than vaccinated cats. The disease was clinically manifested by severe depression, anorexia, ocular discharge, nasal discharge, sneezing, conjunctivitis, keratitis. In addition, some cats have had oral ulceration and fever.Almost all cats with FHV-1 have presented a slight increase of white blood cells (17.77 ± 0.89 103/µl).

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