Breeding Inbred Tomato for Resistance to Yellow Leaf Curl Virus by Marker-Assisted Selection

Received: 10-06-2020

Accepted: 21-01-2021

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Issue: Vol. 19 No. 3 (2021)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Hai, T., Ton, P., Hien, P., & Trung, N. (2024). Breeding Inbred Tomato for Resistance to Yellow Leaf Curl Virus by Marker-Assisted Selection. Vietnam Journal of Agricultural Sciences, 19(3), 399–409. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/804

Breeding Inbred Tomato for Resistance to Yellow Leaf Curl Virus by Marker-Assisted Selection

Tong Van Hai (*) 1 , Phan Huu Ton 2, 1 , Phan Thi Hien 1 , Nguyen Quoc Trung 1

  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 2 Trung tâm Bảo tồn và Phát triển nguồn gen cây trồng, Học viện Nông nghiệp Việt Nam

    • Keywords

    DNA markers, tomato, tomato yellow leaf curl virus, resistant gene

    Abstract


    Tomato yellow leaf curl disease (TYLC) is one of the most dangerous tomato diseases in Vietnam. So far, using resistant genes for breeding tomato is one of the most effective solutions to control this disease. Currently, 5 resistance genes to yellow leaf curl have been identified as Ty1, Ty2, Ty3, Ty4and ty5genes. DNA markers linked to those genes were also discovered that have assisted to select the resistance genes more easily and accurately. In this study, we used TG97 marker to select Ty1resistance gene in F2population (H12 ×AVRDC188) and P6-25 marker to select Ty3 resistance gene in F2population (H12 ×AVRDC195). As a result shown, 7 individuals possess Ty1homozygous resistance gene and 4 individuals possess Ty3homozygous resistance gene were selected. The individuals that carrying resistant genes were mixed together, planted for self-fertilization until the F7generation. The two promising lines were selected including: TP130 line containing Ty1gene and TP135 lines containing Ty3gene. TP130 and TP135 lines were basically tested in three cropping seasons and evaluated their resistance to yellow leaf curl by artificial infection. The results showed that TP130 and TP135 lines had high yield and high resistance to TYLC diseases.

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