Received: 10-06-2020
Accepted: 19-08-2020
DOI:
Views
Downloads
How to Cite:
Study onin vitroApplication of Silver Nanoparticles inLeaf Propagation of Globe Amaranth(Gomphrena globosaL.)
,
Dong Huy Gioi
2
Keywords
Globe Amaranth(Gomphrena globosaL.), nano silver, callus, in vitro propagation
Abstract
Globe amaranth(Gomphrena globosaL.) is one of the beautiful flowers and is popularly grown in Vietnam and many worldwide countries. The objective of this study was to assess the effect of nano silver on the in vitropropagation of Globe Amaranthfrom leaves. The different concentrations of silver nanoparticles (2, 4, 6, 8 and 10ppm) were added to the culture medium at all stages of the tissue culture. The results showed that the callus induction mediumsupplemented with 4-6ppm of nano silver gave the callus formation rate to reach 100%, callus were ivory white, soft and spread across of sample surface after 4 weeks of culturing; the callus of Globe Amaranthwas cultured in shoot regeneration medium supplemented with 4ppm of nano silver for the best shoot generation effect, the shooting rate reached 100%, the average number of shoots was 5.32 shoots/callus, the average height of shoots reached 2.36cm; the highest number of shoots per explant (8.36) was found onshoot multiplication medium supplemented with 6 ppm of nano silver; the root induction mediumadded with 8 ppm of nano silver gave rooting rate of 100%,the average number of roots was 4.08 roots/shoots, the root length reached 4.16cm after 4 weeks of culturing.
References
Amir Ali, Sher Mohammad, Mubarak Ali Khan, Naveed Iqbal Raja, Mohammad Arif, Atif Kamil & Zia-ur-Rehman Mashwani (2019). Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata. Artificial cells, Nanomedicine, and Biotechnology. 47(1): 715-724.
Dương Tấn Nhựt, Nguyễn Bá Nam (2009). Ảnh hưởng của hệ thống chiếu sáng đơn sắc lên sự sinh trưởng và phát triển của cây hoa cúc (Chrysanthemum morifoliumcv. “Nut”) nuôi cấy in vitro. Tạp chí Công nghệ sinh học. 7: 91-98.
Đồng Huy Giới & Ngô Thị Ánh (2017). Nghiên cứu sử dụng chế phẩm nano trong nuôi cấy mô cây mía (Saccharum offcinarumL.). Tạp chí Khoa học Công nghệ Nông nghiệp Việt Nam. 6: 35-40.
Đồng Huy Giới & Dương Thị Mến (2017). Nghiên cứu sử dụng chế phẩm nano trong nuôi cấy mô cây hoa hồng cổ Sapa. Tạp chí Khoa học Công nghệ Nông nghiệp Việt Nam. 5: 59-65.
Hediat M. & Salama H. (2012). Effects of silver nanoparticles in some crop plants. Common bean (Phaseolus vulgarisL.) and corn (Zea maysL.). International research journals of Biotechnology. 3(10): 190-197
Jala A. (2014). Role of 2,4-D on callus induction and shoot formation to increase number of shoot in miniature rose in vitro. American Transaction on Engineering and Applied Sciences. 3(3): 207-213.
Kharrazi M., Nemati H., Tehranifar A., Bagheri A. & Sharifi A. (2011). In vitroculture of carnation (Dianthus caryophyllusL.) focusing on the problem of vitrification. J. Biol. Environ Sci. 13: 1-6.
Liyan Jin, Yang Yang1, Wenjie Gao, Mingxue Gong, Jijia Wang, Neil O. Anderson & Miao He (2017). Establishment of callus induction and cell suspension cultures of Dendrathema indicumvar. Aromaticuma scented chrysanthemum. Journal of Plant Studies. 6(2): 38-44.
Manu Pant, Ankita Lal & Rashi Jain (2015). A simple cost effective method for mass propagation of Chrysanthemum morifolium and antibacterial activity assessment of in vitroraised plantlets. Journal of Applied Pharmaceutical Science. 5(7): 103-111.
Murashige T. & Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Nasser M., Sepideh Z.V. & Sajjad K. (2013). Plant in vitroculture goes nano: Nanosilver-mediated decontamination of ex vitroexplants. Journal of Nanomedicine & Nanotechnology. 4(2): 1-4.
Nabeel K. Al-Ani (2011). Using silver nano- particles to increase efficiency of sterile solution for in vitrotechniques. Iraqi Journal of Cancer and Medical Genetics. 4(1): 48- 51.
Nguyễn Thị Diệu Hương & Dương Tấn Nhựt (2004). Hoàn thiện quy trình nhân nhanh giống cây hoa cúc (Chrysanthemum indicumL.) sạch bệnh bằng kỹ thuật nuôi cấy đỉnh sinh trưởng. Tạp chí Sinh học. 4: 45-48.
Phạm Văn Chương, Hồ Ngọc Giáp, Võ Văn Trung, Lê Thị Thu Hương &Hồ Thị Trang(2016). Sản xuất giống hoa cúc tại bắc trung bộ bằng kỹ thuật nuôi cấy mô. Tạp chí Khoa học và Công nghệ. 6: 1-4.
Rostami A.A. & Shahsavar A. (2009). Nano-silver particles eliminate the in vitrocontaminations of olive ‘Mission’ explants. Journal of Plant Sciences. 8(7): 505-509.
Yesmin S., Hashem A., Das K.C., Hasan M.M. & Islam M.S. (2014). Efficient in vitro regeneration of chrysanthemum (Chrysanthemum morifoliumRamat.) through nodal explant culture. Nuclear Science and Application. 23(1&2): 47-50.