Establishment of in vitroPropagation Protocol for Baikal Skullcap (Scutellaria baicalensisGeorgi.)

Received: 18-05-2020

Accepted: 28-12-2020

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Issue: Vol. 19 No. 3 (2021)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Son, D., Hoang, B., Ninh, N., Phip, N., Khanh, P., Tam, D., … Hai, N. (2024). Establishment of in vitroPropagation Protocol for Baikal Skullcap (Scutellaria baicalensisGeorgi.). Vietnam Journal of Agricultural Sciences, 19(3), 301–310. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/793

Establishment of in vitroPropagation Protocol for Baikal Skullcap (Scutellaria baicalensisGeorgi.)

Dinh Truong Son (*) 1, 2 , Bui Huy Hoang 3 , Nguyen Hai Ninh 3 , Ninh Thi Phip 4 , Pham Ngoc Khanh 5 , Dang Thi Thanh Tam 3 , Nguyen Thi Lam Hai 3 , Nguyen Thanh Hai 3

  • 1 Viện Sinh học Nông nghiệp, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 3 Khoa Công nghệ sinh học,Học viện Nông nghiệp Việt Nam
  • 4 Khoa Nông học,Học viện Nông nghiệp Việt Nam
  • 5 Trạm Nghiên cứu trồng cây thuốc Sa Pa - Viện Dược liệu,Thịxã Sa Pa - Lào Cai

    • Keywords

    Baikal skullcap, Scutellaria baicalensisGeorgi., plant tissue culture, in vitro

    Abstract


    Baikal skullcap (Scutellaria baicalensisGeorgi.),a member of the Lamiaceae familyhas been commonly used in traditionalmedicine. Baikal skullcap (called“Hoang cam” in Vietnamese) was introduced into Vietnam and has been planted in some areasin this country. Due to its naturally slow growth and development, large growing areas have not been developed yet. Presently, the most common method of propagation of Baikal skullcap isusingits seeds. Thisstudywas conducted toestablish a rapid micropropagation protocol that can beappliedfor multiplicationof elite strainsand actively provide the disease-free seedlings. The seeds were sterilized using0.5% Presept solution for 15 min, then placed on a medium containing water, sucrose, and agar for germination. Microshoots were highly induced and multiplied (7.47 ± 0.57 shoots/explant) on MS medium supplemented with 1mg/l BA. The suitable sucrose concentrations for the growth and development of microshoots were 20 g/l or 30 g/l. Allshoots were rootedby 100% by addingMS medium supplemented with 0.8-1.6 mg/l IBA. Therefore, theestablished protocol can be applied forin vitro micropropagationofBaikal skullcap.

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