Production of Polyclonal Antiserum using Recombinant Coat Protein of Passiflora mottle virus Infecting Passion Fruit in Vietnam

Received: 28-09-2020

Accepted: 16-12-2020

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Issue: Vol. 19 No. 2 (2021)

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NÔNG HỌC

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Cuong, H., Tuyet, L., Ha, T., Huy, N., & Dung, D. (2024). Production of Polyclonal Antiserum using Recombinant Coat Protein of Passiflora mottle virus Infecting Passion Fruit in Vietnam. Vietnam Journal of Agricultural Sciences, 19(2), 195–205. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/788

Production of Polyclonal Antiserum using Recombinant Coat Protein of Passiflora mottle virus Infecting Passion Fruit in Vietnam

Ha Viet Cuong (*) 1, 2, 3 , Le Thi Tuyet 2 , Tran Nguyen Ha 2 , Nguyen Duc Huy 2 , Do Tan Dung 2

  • 1 Trung tâm Nghiên cứu Bệnh cây Nhiệt đới, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Nông học, Học viện Nông nghiệp Việt Nam
  • 3 Bộ môn Bệnh cây, Khoa Nông học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Passifloramottlevirus, potyvirus, DIBA, ELISA, passionfruit, Vietnam

    Abstract


    ThemajoraimofthisworkwastoproducepolyclonalantibodiesfordiagnosisofPassifloramottlevirus(PaMoV),aputativenovelpotyvirus(thegenusPotyvirus,thefamilyPotyviridae),whichwasfirstdiscoveredfrompassionfruit(Passifloraedulis)andnamedinVietnam.Theentiregeneencodingcoatprotein(CP)ofPaMoVwasclonedonpET28avectorandtheexpressionofthisproteinwasevaluatedintheEscherichiacoli(E.coli)strainRosetta(DE3).ThedenaturedrecombinantPaMoVCPproteinwasusedasan antigenforrabbitinjection.ThetiterandspecificityoftherabbitantiserumwereevaluatedbyDot-ImmunobindingAssay(DIBA)andPlateTrappedAntigenEnzyme-LinkedImmunosorbentAssay(PTA-ELISA).Theexperimental resultsshowed: (i) Rosetta(DE3)wasasuitablehoststrainforexpressionofthePaMoVCPprotein, (ii) thePaMoV CP antiserumdidnotreactwithhealthypassionfruitsapanddetected easily cognate inpassionfruitleaves, and (iii) the antiserumalsoreactedwithtwopotyviruses,Telosmamosaicvirus(TelMV)andEastAsianpassifloravirusstrainIB(EAPV-IB),bothareinfectingpassionfruitinVietnam.

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