Effects of Cooling Methods on Sperm Quality in Boar Semen Preservation at Low Temperature

Received: 31-08-2020

Accepted: 08-12-2020

DOI:

Views

0

Downloads

0

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Doanh, B., Yen, D., Thu, C., Kien, N., Le, N., Hai, D., & Dang, P. (2024). Effects of Cooling Methods on Sperm Quality in Boar Semen Preservation at Low Temperature. Vietnam Journal of Agricultural Sciences, 19(2), 246–253. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/782

Effects of Cooling Methods on Sperm Quality in Boar Semen Preservation at Low Temperature

Bui Huy Doanh (*) 1 , Dinh Thi Yen 1 , Cu Thi Thien Thu 1 , Nguyen Ngoc Kien 2 , Nguyen Thi Tuyet Le 1 , Dang Thai Hai 1 , Pham Kim Dang 1

  • 1 Khoa Chăn nuôi, Học viện Nông nghiệp Việt Nam
  • 2 Công ty CP Giống gia súc Hà Nội
  • Keywords

    Semen, preservation, semenquality, temperature

    Abstract


    This study was conducted on 720 semen samples of six Duroc boars to evaluate the effects of the different cooling methods on semen quality when stored at low temperatures in an extender without antibiotics. Semen was diluted in a modified BTS extender without antibiotics. Semen samples were cooled from 30°C to 5°C using five different cooling rates before stored at 5°C. Diluted semen was investigated after 24h, 48h, 72h and 120h of preservation. The results showed that: the lowest cooling rate sample (5-A4: 2h at room temperature, then stored at 10°C for 3 h) had sperm motility, abnormal acrosome, as well as morphology in different days of storage, which did not differ from semen samples stored at 17°C (P >0.05). Rapid cooling rate and direct storage at 5°C (5-A0 stored immediately at 5°C after dilution) were reduced sperm motility as well as increased abnormal spermatozoa (P <0.05). Time storage has influenced sperm motility (P <0.05). Therefore, an optimal cooling rate (slowly cooling rate at room temperature and 10°C before stored at 5°C) for hypothermic storage was established for antibiotic-free storage at 5°C.

    References

    Auroux M.R.,JacquesL.,MathieuD.&AuerJ.(1991).Isthespermbacterialratioadeterminingfactorinimpairmentofspermmotility:aninvitrostudyinmanwithEscherichia coli.InternationalJournalofAndrology.14(4):264-270.

    BộKhoahọcvàCôngnghệ(2011).TiêuchuẩnquốcgiaTCVN9111:2011vềLợngiốngngoại-Yêucầukỹthuật.

    Buhr M.M., Canvin A.T. & Bailey J.L.(1989).Effectsofsemenpreservationonboarspermatozoaheadmembranes.GameteRes.23(4):441-9.

    De Leeuw F.E., Chen H.C., Colenbrander B. & Verkleij A.J.(1990).Cold-inducedultrastructuralchangesinbullandboarspermplasmamembranes.Cryobiology.27(2):171-83.

    Drobnis E.Z., Crowe L.M., Berger T., Anchordoguy T.J., Overstreet J.W. & Crowe J.H.(1993).Coldshockdamageisduetolipidphasetransitionsincellmembranes:ademonstrationusingspermasamodel.JExpZool.265(4):432-7.

    El-Mulla K.F., Köhn F.M., Dandal M., El Beheiry A.H., Schiefer H.G., Weidner W. & Schill W.B.(1996).InvitroeffectofEscherichiacolionhumanspermacrosomereaction.ArchAndrol.37(2):73-8.

    HàXuânBộ,Đỗ Đức Lực &Đặng Vũ Bình (2011).ĐánhgiáphẩmchấttinhdịchlợnPiétrainkhángstressnhậptừbỉnuôitạiXínghiệpChănnuôiĐồngHiệp-HảiPhòng.TạpchíKhoahọcvàPháttriển.9(5):7.

    HàXuânBộ,Đỗ Đức Lực,Bùi Văn Định,Bùi Hữu Đoàn,Vũ Đình Tôn&Đặng Vũ Bình(2013).KhảnăngsinhtrưởngvàphẩmchấttinhdịchlợnđựcPiétrainkhángstressnuôitạiTrungtâmGiốnglợnchấtlượngcao-TrườngĐạihọcNôngnghiệpHàNội.TạpchíKhoahọcvàPháttriển.11(2):6.

    Harrison R.A., Dott H.M. & Foster G.C.(1978).Effectofionicstrength,serumalbuminandothermacromoleculesonthemaintenanceofmotilityandthesurfaceofmammalianspermatozoainasimplemedium.JReprodFertil.52(1):65-73.

    Johnson L.A.,WeitzeK.F., Fiser P. & Maxwell W.M.(2000).Storageofboarsemen.AnimReprodSci.62(1-3):143-72.

    Martín L.O., Muñoz E.C.,DeCupereF.,VanDriesscheE.,Echemendia-Blanco D., Rodríguez J.M.&BeeckmansS.(2010).Bacterialcontaminationofboarsemenaffectsthelittersize.AnimReprodSci.120(1-4):95-104.

    NesciS.,SpinaciM.,GaleatiG.,NerozziC.,PagliaraniA.,AlgieriC.,TamaniniC.&BucciD.(2020).Spermfunctionandmitochondrialactivity:Aninsightonboarspermmetabolism.Theriogenology.144:82-88.

    Nguyen Q.T.,WallnerU.,SchmickeM.,WaberskiD.&HenningH.(2016).EnergymetabolicstateinhypothermicallystoredboarspermatozoausingarevisedprotocolforefficientATPextraction.BiologyOpen.10.1242/bio.017954.

    PaulenzH.,GrevleI.,TverdalA.,HofmoP.&BergK.A.(1995).PrecisionoftheCoulter®CounterforRoutineAssessmentofBoar-spermConcentrationinComparisonwiththeHaemocytometerandSpectrophotometer.ReproductioninDomesticAnimals.30(3):107-111.

    Payne B.J.,ClarkS.&MaddoxC.(2008).Achromobacterxylosoxidansinextendedsemencausesreproductivefailureinartificiallyinseminatedsowsandgilts.JournalofSwineHealthandProduction.16(6):316-322.

    Pursel V.G., Schulman L.L. & Johnson L.A.(1973).Effectofholdingtimeonstorageofboarspermatozoaat5C.JAnimSci.37(3):785-9.

    SchmidS.,HenningH.,OldenhofH., Wolkers W.F., Petrunkina A.M.&WaberskiD.(2013a).Thespecificresponsetocapacitatingstimuliisasensitiveindicatorofchillinginjuryinhypothermicallystoredboarspermatozoa.Andrology.1(3):376-86.

    SchmidS.,HenningH., Petrunkina A. M., Weitze K F.&WaberskiD.(2013b).Responsetocapacitatingstimuliindicatesextender-relateddifferencesinboarspermfunction.JAnimSci.91(10):5018-25.

    Schulze M., Grobbel M., Riesenbeck A., Brüning S., Schaefer J., Jung M. & Grossfeld R. (2017). Dose rates of antimicrobial substances in boar semen preservation time to establish new protocols. Reproduction in Domestic Animals.52(3): 397-402.

    SchulzeM.,HenningH.,RüdigerK.,WallnerU.&WaberskiD.(2013).Temperaturemanagementduringsemenprocessing:Impactonboarspermqualityunderlaboratoryandfieldconditions.Theriogenology.80(9):990-998.

    SepúlvedaL.,BussalleuE.,YesteM.&BonetS.(2014).EffectsofdifferentconcentrationsofPseudomonasaeruginosaonboarspermquality.AnimReprodSci.150(3-4):96-106.

    SpeckS.,CourtiolA.,JunkesC.,DatheM.,MüllerK.&SchulzeM.(2014).Cationicsyntheticpeptides:assessmentoftheirantimicrobialpotencyinliquidpreservedboarsemen.PLoSOne.9(8):e105949-e105949.

    UbedaJ.L.,AusejoR.,DahmaniY.,FalcetoM.V.,UsanA.,MaloC.&Perez-MartinezF.C.(2013).AdverseeffectsofmembersoftheEnterobacteriaceaefamilyonboarspermquality.Theriogenology.80(6):565-70.

    WaberskiD.,HenningH.&PetrunkinaA.(2011).Assessmentofstorageeffectsinliquidpreservedboarsemen.ReproductioninDomesticAnimals.46(s2):45-48.

    WaberskiD.,LutherA.M.,GrüntherB.,JäkelH.,HenningH.,Vogel C., Peralta W. & Weitze K.F.(2019a).Spermfunctioninvitroandfertilityafterantibiotic-free,hypothermicstorageofliquidpreservedboarsemen.ScientificReports.9(1):14748.

    WaberskiD.,RiesenbeckA.,SchulzeM.,WeitzeK.F.&JohnsonL.(2019b).Applicationofpreservedboarsemenforartificialinsemination:Past,presentandfuturechallenges.Theriogenology.137:2-7.

    Weitze K.F. (2011). Theimportanceofboarspermmotilityandmorphologyforfertility,InternationalPigTopics,27(5): 13-15.

    WolffH.,PanhansA.,StolzW.&MeurerM.(1993).AdherenceofEscherichiacolitosperm:amannosemediatedphenomenonleadingtoagglutinationofspermandE.coli.FertilSteril.60(1):154-8.

    YesteM.(2015).RecentAdvancesinBoarSpermCryopreservation:StateoftheArtandCurrentPerspectives.ReprodDomestAnim.50(Suppl2):71-9.