Received: 31-08-2020
Accepted: 08-12-2020
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Effects of Cooling Methods on Sperm Quality in Boar Semen Preservation at Low Temperature
Keywords
Semen, preservation, semenquality, temperature
Abstract
This study was conducted on 720 semen samples of six Duroc boars to evaluate the effects of the different cooling methods on semen quality when stored at low temperatures in an extender without antibiotics. Semen was diluted in a modified BTS extender without antibiotics. Semen samples were cooled from 30°C to 5°C using five different cooling rates before stored at 5°C. Diluted semen was investigated after 24h, 48h, 72h and 120h of preservation. The results showed that: the lowest cooling rate sample (5-A4: 2h at room temperature, then stored at 10°C for 3 h) had sperm motility, abnormal acrosome, as well as morphology in different days of storage, which did not differ from semen samples stored at 17°C (P >0.05). Rapid cooling rate and direct storage at 5°C (5-A0 stored immediately at 5°C after dilution) were reduced sperm motility as well as increased abnormal spermatozoa (P <0.05). Time storage has influenced sperm motility (P <0.05). Therefore, an optimal cooling rate (slowly cooling rate at room temperature and 10°C before stored at 5°C) for hypothermic storage was established for antibiotic-free storage at 5°C.
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