Received: 30-07-2020
Accepted: 08-09-2020
DOI:
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Application of Immunohistochemistry to Detect Antigen of African Swine Fever Virus in Infected Pigs
,
Truong Quang Lam
1
,
Hoang Thi Thu Hien
1
,
Nguyen Huu Nam
1
,
Lai Thi Lan Huong
1
,
Bui Tran Anh Dao
1
,
Yamaguchi Ryoji
2
,
Nguyen Thi Lan
1
Keywords
African swine fever, antigen, immunohistochemistry
Abstract
The study was conducted to detect antigen of African swine fever virus (ASFV) in the tissues of infected pigs, therefore, contributing to clarifying the pathogenesis of the virus and providing a scientific basis for the selection of disease samples suitable for research and diagnostics. Nine organs of 5 ASFV positive pigs were collected and stained immunohistochemistry to detect viral antigen. Resultsshowed that the ASF virus antigen was highly concentrated in organs such as lymph nodes and spleen, the lungs, liver and kidney; followed by brain, heart, intestines and stomach.Antigenwas detected in macrophage cells, mononuclear cells in many different organs, hepatocytes and renal tubular epithelial cells.
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