Molecular Biological Characteristics of Canine Distemper Virus Strain Isolated from Some Provinces in TheNorth of Vietnam

Received: 06-02-2016

Accepted: 13-03-2016

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Issue: Vol. 15 No. 1 (2017)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Nen, T., Lan, N., Thanh, N., & Hung, L. (2024). Molecular Biological Characteristics of Canine Distemper Virus Strain Isolated from Some Provinces in TheNorth of Vietnam. Vietnam Journal of Agricultural Sciences, 15(1), 44–57. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/335

Molecular Biological Characteristics of Canine Distemper Virus Strain Isolated from Some Provinces in TheNorth of Vietnam

Tran Van Nen (*) 1 , Nguyen Thi Lan 1 , Nguyen Van Thanh 1 , Luong Quoc Hung 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Canine distemper virus, molecular biological characteristics, Vietnam

    Abstract


    The study was conducted to investigate molecular biological characteristics of isolated canine distemper virus (CDV) strains from some provinces in the North of Vietnam. Five virus strains in this study were isolated from clinical samples of dogs infected with CDV in Nam Dinh, Thai Binh, Hung Yen, Hanoi and BacGiang provinces in 2015. After the viral ARNs were extracted from tissue samples, RT-PCR and PCR-sequence assay were performed using specific primer pairs to amplify haemagglutinin (H) and phosphoprotein (P) genes of CDV. The products of PCR-sequence were directly sequenced using Sanger sequencing method. The sequence of H and P genes was 1824bp and 402bp in length, respectively. The high similarity of nucleotides in H and P genes between 5 virus strains was 90.05 - 99.61% and 94.81 - 99.75%, respectively. The high similarity of amino acids encoded by H and P genes between 5 virus strains was high with 89.38 - 99.5% and 92.47 - 100.0%, respectively. The phylogenetic trees showed that five isolated virus strains belonged to three different genotypes: Asia 1, Asia 2 and Classic. These results indicated that pathogenic genotypes of virus strains were circulating in northernprovinces of Vietnam. These results are useful information for producing vaccine from pathogenic virus strains to control CDV in Vietnam.

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