IDENTIFICATION OF NOVEL T CELL EPITOPE REPERTOIRE IN NSP1 AND GP3 OF THE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS TYPE 2

Received: 02-11-2015

Accepted: 03-05-2016

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Issue: Vol. 14 No. 4 (2016)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Giang, T., Dien, P., Hieu, D., & Chung, W. (2024). IDENTIFICATION OF NOVEL T CELL EPITOPE REPERTOIRE IN NSP1 AND GP3 OF THE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS TYPE 2. Vietnam Journal of Agricultural Sciences, 14(4), 613–619. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/272

IDENTIFICATION OF NOVEL T CELL EPITOPE REPERTOIRE IN NSP1 AND GP3 OF THE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS TYPE 2

Tran Thi Huong Giang (*) 1 , Phan Hong Dien 2 , Dong Van Hieu 3 , Wen Bin Chung 2

  • 1 Faculty of Veterinary Medicine, Vietnam National University of Agriculture
  • 2 Department of Veterinary Medicine, National Pingtung University of Science and Technology
  • 3 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    PRRSV, Elispot, IFN-γ, nhóm quyết định kháng nguyên

    Abstract


    Virus gây hội chứng rối loạn sinh sản và hô hấp ở lợn (PRRSV) đã trở thành một trong những căn bệnh chính ảnh hưởng lớn tới ngành chăn nuôi lợn trên thế giới. Một số vắc xin đã được sử dụng để phòng và làm giảm ảnh hưởng của PRRS, tuy nhiên căn bệnh vẫn tồn tại và gây ảnh hưởng tới các trang trại chăn nuôi lợn. Phát triển một loại vắc xin trên cơ sở những hiểu biết về cơ chế miễn dịch qua trung gian tế bào nhằm kiểm soát PRRS là việc làm cấp bách và cần thiến hiện nay. Trong nghiên cứu này, chúng tôi đã sử dụng kỹ thuật tin sinh học để xác định các nhóm kháng nguyên tế bào T dựa trên chủng PRRSV ATCC VR-2332 (U87392.3). 12 trình tự a xít amin được sàng lọc bằng kỹ thuật IFN-γ ELISpot trong đáp ứng với tế bào PBMCs. Kết quả đã xác định được 2 peptide gồm 1b3 (YQLASYASYI) và GP3 (SVYAWLAFLSFSY) là epitop tế bào T. Đây có thể là một thông tin có giá trị trong việc phát triển một loại vắc xin có hiệu quả phòng PRRSV.

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