Genetic Diversity in Common Bean Accessions Evaluated by Means of Morpho-Agronomical and SSR Data

Received: 12-05-2016

Accepted: 20-12-2016

DOI:

Views

2

Downloads

0

Section:

NÔNG HỌC

How to Cite:

Ngoc, P., Trung, N., & Liet, V. (2024). Genetic Diversity in Common Bean Accessions Evaluated by Means of Morpho-Agronomical and SSR Data. Vietnam Journal of Agricultural Sciences, 14(12), 1874–1885. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1500

Genetic Diversity in Common Bean Accessions Evaluated by Means of Morpho-Agronomical and SSR Data

Pham Thi Ngoc (*) 1 , Nguyen Quoc Trung 2 , Vu Van Liet 1

  • 1 Khoa Nông học, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • Keywords

    Common bean (Phaseolus vulgaris L.), genetic diversity, SSR marker, morphological marker

    Abstract


    The knowledge and understanding of genetic variability of common bean (Phaseolus vulgarisL.) germplasm is important for utilization and conservation. The Oobjective of this study was to analyzesis ofgenetic diversity of 60 accessions that collected in Vietnam and from other countries. Results based on mMorphological markers showed that 60 germplasms accessions werearevery diverse and they were dievided into 7 groups with genetic similarity index of 0.17. Using SSR markers to evaluate genetic diversity,Fifteen15SSR markersamong 20 used wereshowed polymorphismcwith total of 69 alleleswith and an average of 4.6 alleles per locus. BM152 marker had highest PIC value (0.73). Four major clusters were grouped for 60 accessions with genetic similarity at 0.69. The,dendrogram showing the genetic relationships was constructed by the unweighted pairedgroup method with arithmetic average (UPGMA) using the software NTSYS-pc 2.01. The results of this study indicatedthat SSR analysis could be successfully used for the estimation of genetic diversity among common bean accessions. The diversity of common beanThatare of the highest usefulnessfor breeding purposes.

    References

    Asfaw A., M.W. Blair, S.E. Beebe, I.M. Rao, M.J Devi, and J. Polania (2013). Common beans, biodiversity, and multiple stresses: challenges of drought resistance in tropical soils, Crop and Pasture Science, 65(7): 667 - 675.

    Benchimol L.L, T. Campos, S.A.M. Carbonell, C.A. Colombo, A.F. Chioratto, E.F. Formighieri, A.P. Souza (2007). Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins using new developed microsatellite markers. Genet Resour Crop Evol., 54: 1747 - 1762.

    Blair M.W., F. Pedraza, H.F. Buendia, E. Gaitan - Solis (2003). Development of a genome - wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theor. Appl. Genet., 107: 1362 - 1374.

    Blair,M.W., M.C. Giraldo, H.F. Buendia, E. Tovar, M.C. Duque, S.E. Beebe (2006). Microsatellite marker diversity in common bean (Phaseolus vulgaris L.). Theor. Appl. Genet., 113: 100 - 109.

    Boros L., A. Wawer, K. Borucka (2014). Morphological, Phenological and agronomical characterization of variability among common bean (Phaseolus vulgaris L.) local populations from the national centre for plant genetic resources Polish Genebank, Journal of Horticultural Research, 22(2): 123 - 130.

    Ceylan A., Öcal N, Akbulut M (2014). Genetic diversity among the Turkish common bean cultivars (Phaseolus vulgaris L.) as assessed by SRAP, POGP and cpSSR markers, Biochemical Systematics and Ecology, 54: 219 - 229.

    Doyle, J.J. and Doyle J.L. (1987). A rapid DNA isolationprocedure for small quantities of fresh leaf tissue. Phytochem Bull., 19: 11 - 15.

    Freitas G., J.osé F. T. Ganança, H. Nóbrega, E. Nunes, G. Costa, J.J. Slaski, M.A.A. Pinheiro de Carvalho (2011). Morphological evaluation of common bean diversity on the Island of Madeira, Genetic Resources and Crop Evolution, 58(6):861 - 874.

    Gaitan - Solis E., M.C. Duque, K.J. Edwards, J. Tohme (2002). Microsatellite repeats in common bean (Phaseolus vulgaris): Isolation, characterization, and cross - species amplification in Phaseolus ssp. Crop Sci., 42: 2128 - 2136.

    Gómez O.J, M.W Blair, B.E Frankow - Lindberg, U Gullberg (2004). Molecular and Phenotypic Diversity of Common Bean Landraces from Nicaragua. Crop sci. Society of America, 44: 1412 - 1418.

    Khaidiza M.I., K. Haliloglu, E. Elkoca, M. Aydin, F. Kantar (2012). Genetic Diversity of Common Bean (Phaseolus vulgaris L.) Landraces Grown in Northeast Anatolia of Turkey Assesessed with Simple Sequence Repeat Markers. Turkish Journal of Field Crop, 17(2): 145 - 150.

    Kumar A., P.K Singh, N. Rai, G.P Bhaskar, D. Datta (2014). Genetic diversity of French bean (Phaseolus vulgaris L.) genotypes on the basis of morphological traits and molecular markers. Indian Journal of Biotechnology, 13: 207 - 213.

    Nei M. and W.H. Li (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sct. USA, 76(10): 5269 - 5273.

    Okii D, P. Tukamuhabwa, T. Odong, A. Namayanja, J. Mukabaranga, P. Paparu, P. Gepts (2014). Morphological diversity of tropcal common bean germplasm. African Crop Science Journal, 22(1): 59 - 67.

    Raggi L, Barbara. Tiranti, Valeria. Negri (2012). Italian common bean landraces: diversity and population structure, Genetic Resources and Crop Evolution, 60(4): 1515 - 1530.

    Rohlf F.J (2000). NTSYSpc Numerical Taxonomy and Multivariate Analysis System Version 2.1.

    Schmutz J., P. E Mc Clean, S. Mamidi, G. A. Wu1, S. B. Cannon, J. Grimwood, J. Jenkins, S. Shu, Q. Song, C. Chavarro, M. Torres - Torres, V. Geffroy, S. M. Moghaddam, D. Gao, B. Abernathy, K. Barry, M. Blair, M. A. Brick, M. Chovatia, P. Gepts, D. M. Goodstein, M. Gonzales, U. Hellsten, D. L. Hyten, G. Jia, J. D. Kelly, D. Kudrna, R. Lee, M. M. S. Richard, P. N. Miklas, J. M. Osorno, J. Rodrigues, V. Thareau, C. A. Urrea, M. Wang, Y. Yu, M. Zhang, R. A. Wing, P. B. Cregan, D. S. Rokhsar and S. A. Jackson (2014). A reference genome for common bean and genome - wide analysis of dual domestications, Nature Genetics, 46(7).

    Wang A., Y. Ding, Z. Hu, C. Lin, S. Wang, B. Wang, H. Zhang and G. Zhou (2012). Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome. Int. J. Mol. Sci., 13, 11188 - 11193.

    Yu K., S.J. Park, V. Poysa (1999). Abundance and variation of microsatellite DNA sequences in beans (Phaseolus and Vigna). Genome, 42: 27 - 34.

    Yu K., S.J. Park, V.Poysa and P.Gepts (2000). Intergration of Simple Sequence Repeat (SSR) Markers Into a Molecular Linkage Map of Common Bean (Phaseolus vulgaris L.). American Genetic Association, 91: 429 - 434.

    Zhang X., M.W. Blair, S. Wang (2008). Genetic diversity of Chinese common bean (Phaseolus vulgaris L.) landraces assessed with simple sequence repeat markers. Theoretical and Applied Genetics, 117(4): 629 - 40.