Influence of Storage Conditionson Pollen Germinability of someMelonCultivars

Received: 12-12-2022

Accepted: 05-01-2024

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Anh, N., Hoa, N., Tuan, N., Tung, N., Hoang, P., & Anh, B. (2024). Influence of Storage Conditionson Pollen Germinability of someMelonCultivars. Vietnam Journal of Agricultural Sciences, 22(3), 285–293. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1292

Influence of Storage Conditionson Pollen Germinability of someMelonCultivars

Nguyen Tuan Anh (*) 1 , Nguyen Thi Hoa 1 , Nguyen Thanh Tuan 1 , Nguyen Thanh Tung 2 , Pham Van Hoang 1 , Bui Phuong Anh 1

  • 1 Khoa Nông học, Học viện Nông nghiệp Việt Nam
  • 2 Viện Nghiên cứu và Phát triển cây trồng, Học viện Nông nghiệp Việt Nam
  • Keywords

    Melon, Cucumis melo, pollen viability, pollen germinability, relative humidity, temperature, organic solvent

    Abstract


    Melon (Cucumis melo) is a crop of economic value and suitable for growing in greenhouses in North Vietnam. In the present study, we examined the influenceof storage conditions on thepollen germinability of introduced melon cultivars. The research aimed to determine the optimal conditions to preserve pollensfor application in the production, breeding, and selection of melons. Pollen grains of 4 melon cultivars, i.e.Bach Ngoc, Kim Hoang Hau, Gia Huy, and Makuwa were collected and preserved under different temperatures (25C, 8C, and -20C), relative humidities (20%, 40%, 60%), and organic solvents (acetone, n-hexane, cyclohexane, diethyl ether, and absolute alcohol). Pollen germination rates were tested in vitroin 1% agar medium containing 15% sucrose and 5 ppm boric acidfor3 to 30 days depending on experiments. Results showed that when dried and kept under -20C and 20% relative humidity, melon pollen grains sustainedtheir germinabilityat approx 50% at 30 daysafter storage.

    References

    Aronne G. (1999). Effects of relative humidity and temperature stress on pollen viability of Cistus incanusand Myrtus communis. Grana. 38(6): 364-367. doi.org/10.1080/00173130050136154.

    Ćalić Dušica, Jelena Milojević, Maja Belić, Rade Miletić & Snežana Zdravković-Korać (2021). Impact of storage temperature on pollen viability and germinability of four serbian autochthon apple cultivars. Frontiers in Plant Science.12. doi.org/10.3389/fpls.2021.709231.

    Du Guangcong, Jinguang Xu, Chengrong Gao, Jie Lu, Qian Li, Jie Du, Mengwen Lv & Xia Sun (2019). Effect of low storage temperature on pollen viability of fifteen herbaceous peonies. Biotechnology Reports21(March): e00309. doi.org/10.1016/j.btre.2019.e00309.

    García Cruzatty, Rivero L.M. & Droppelmann F. (2015). Effect of temperature and drying on the longevity of stored Nothofagus alpinapollen. New Zealand Journal of Botany.53(3): 155-64. doi.org/10.1080/0028825X.2015.1045528.

    Gok P. Yetisir, Halit Solmaz, Ilknur Sari & Nebahat Eti (2007). Pollen viability and germination rates of 45 watermelon genotypes. Acta horticulturae. 731: 99-102. doi.org/10.17660/ActaHortic.2007.731.13.

    Iwanami Y. & Nakamura N. (1972). Storage in an organic solvent as means for preserving viability of pollen grains. Stain Technology.47(3): 137-39. doi.org/10.3109/10520297209116468.

    Jain Ajay & Shivanna K.R. (1988a). Storage of pollen grains in organic solvents: Effects of solvents on pollen viability and membrane Integrity. Journal of Plant Physiology.132(4): 499-501. doi.org/10.1016/S0176-1617(88)80070-1.

    Jain Ajay & ShivannaK.R. (1988b). Storage of pollen grains in organic solvents: Effect of organic solvents on leaching of phospholipids and its relationship to pollen viability. Annals of Botany.61(3): 325-330. doi.org/10.1093/oxfordjournals.aob.a087561.

    Nepi Massimo, Cresti Mauro, Guarnieri Massimo & Pacini Ettore (2010). Effect of relative humidity on water content, viability and carbohydrate profile of Petunia hybridaand Cucurbita pepopollen. Plant Systematics and Evolution. 284(1): 57-64. doi:10.1007/s00606-009-0237-x.

    PhuongNguyen Tran Dong, Sang Mai The (2021). Evaluation of the condition for storing pollen grains of Japanese melon Cucumis melo L. (Cucurbitaceae). Academia Journal of Biology, 43(3): 87-94. doi.org/10.15625/2615-9023/16161.

    Olmo H.P. (1942). Storage of grape pollen. Proceedings of the American Society for Horticultural Science. 41: 219-224. Retrieved from https://ia802901.us.archive.org/ 2/items/in.ernet.dli.2015.240678/2015.240678.Proceedings-Of.pdf on Sep 20, 2022.

    Visser T. (1955). Germination and storage of pollen [PhD, Wageningen]. Retrieved from https://library.wur.nl/WebQuery/wurpubs/525569. on Sep 20, 2022.

    Whitehead R. (1962). Room-temperature storage of coconut pollen. Nature. 196: 190. doi.org/10.1038/196190a0.