Determination of Optimal Culture Conditions for Mycelial Growth of Black Lingzhi (Amauroderma subresinosum) Strain Am-4

Received: 15-08-2023

Accepted: 25-12-2023

DOI:

Views

2

Downloads

0

Issue: Vol. 21 No. 12 (2023)

Section:

NÔNG HỌC

How to Cite:

Ha, T., Chi, N., & Giang, N. (2024). Determination of Optimal Culture Conditions for Mycelial Growth of Black Lingzhi (Amauroderma subresinosum) Strain Am-4. Vietnam Journal of Agricultural Sciences, 21(12), 1647–1658. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1235

Determination of Optimal Culture Conditions for Mycelial Growth of Black Lingzhi (Amauroderma subresinosum) Strain Am-4

Tran Thu Ha (*) 1 , Nguyen Ham Chi 1 , Nguyen Van Giang 1

  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Black lingzhi, Amauroderma subresinosum, mycelial growth, nutrient sources

    Abstract


    Black Lingzhi, Amauroderma subresinosumof the the family Ganoderma, contains numerous bioactive compounds, such as sterols, flavonoids, phenols, fatty acids, amino acids, polysaccharides, and triterpenes. Whileactive constituents, distribution, and morphological characteristics of black lingzhi have been studied in Vietnam, there has been limited research conducted to determine the ideal culture conditions that promote mycelial growth. This study aimed to determine the optimal culture conditions for mycelialgrowthof Amauroderma subresinosumstrain Am-4, collected from Bao Lam, Lam Dong province. Strain Am-4 was cultured on Ohta, PDA, SDA, MEA, MCM, and Czapek media under different pH levels, temperatures, carbon, nitrogen, and mineral salt sources. Our results indicated that strain Am-4 exhibited rapid mycelialgrowth on MEA medium at 25°Cand pH5. The strain showed the highest growth rate when cultured in a medium containing glucose and NaNO3as the carbon and nitrogen sources, respectively, with the optimal glucose/NaNO3ratio being 10:1. Additionally, ZnSO4.7H2O was found tobetheoptimal mineral salt source. Under the optimized culture conditions, strain Am-4 exhibited fast mycelial growth with a compact density.

    References

    Chandra A. & Purkayastha R.P. (1977). Physiological studies on Indian mushrooms. Transactions of the British Mycological Society. 69: 63-70.

    Dong C.H. & Yao Y.J. (2005). Nutritional requirements of mycelial growth of Cordyceps sinensisin submerged culture. Journal of Applied Microbiology. 99: 483-92.

    El-Nour H.H.A. & Ibraheim A.M. (2021). Effect of some Organic Applications on Biological Efficiency and Productivity of Mushroom (Pleurotus columbines) Grown under Uncontrolled Conditions. Journal of Plant Production. 12(5): 495-503.

    Garraway O.M. & Evans C.R. (1984). Fungal Nutrition and Physiology. John Wiley and Sons, New York.

    Gonkhom D., Luangharn T., Hyde K.D., Stadler M. & Naritsada Thongklang N. (2022). Optimal conditions for mycelial growth of medicinal mushrooms belonging to the genus Hericium. Mycological Progress. 21: 82.

    Giaijuan Q., Shanshan W., Quingjun C., Haolin Z. & Guoquing Z. (2015). Isolation, identifcation, and biological characteristics of a wild Amauroderma mushroom. Chinese Journal of Applied and Environmental. 21(3): 464-469.

    HoaH.T. & WangC.L. (2015). The effects of temperature and nutritional conditions on mycelium growth of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology. 43(1): 14-23.

    Jayasinghe C., Imtiaj A., Hur H., Lee G.W., Lee T.S. & Lee U.Y. (2008) Favorable culture conditions for mycelial growth of Korean wild strains in Ganoderma lucidum. Mycobiology. 36(1): 28-33.

    Jiao C., Xie Y.Z., Yang X., Li H., Li X.M. & Pan H.H. (2013). Anticancer Activity of Amauroderma rude. PLoS One. 8(6): 1-13.

    Jo W.S., Cho Y.J., Cho D.H., Park S.D., Yoo Y.B. & Seok S.J. (2009). Culture conditions for the mycelial growth of Ganoderma applanatum. Mycobiology. 37: 94-102.

    Jo W.S., Kim D.G., Seok S.J., Jung H.Y. & Park S.C. (2014). The culture conditions for the mycelial growth of Auricularia auricula-judae. Journal of Mushrooms. 12(2): 88-95.

    Kumar V., Mishra S.K. & Kaur M. (2019). Effect of different media, temperature and pH on radial mycelial growth of Lentinula edodes strain Le-17-04. Journal of Pharmacognosy and Phytochemistry. 8(1): 345-348.

    Laursen A. (2018). The Effect of Different Nitrogen Sources on Mycelial Growth of Oyster Mushroom, Pleurotus ostreatus. Bachelor Project in Biology. SLU- Department of Biosystems and Technology. 61p.

    Lê Xuân Thám, Nguyễn Lê Quốc Hùng, Đặng Ngọc Quang & Đạo Thị Lương (2009). Phân tích loài nấm Linh chi đen mới phát hiện được ở Vườn Quốc gia Cát Tiên, Đồng Nai - Lâm Đồng. Tạp chí Sinh học. 31(4): 55-64.

    Lee E.J, Park H.S., Lee C.J., Kong W.S. & Koo C.D. (2019). Suitable Conditions for Mycelial Culture of Tremella fuciformis. The Korean Journal of Mycology. 47: 1-12.

    Letti L.A., Vítola F.M., Pereira G.V., Karp S.G., Medeiros A.B., Costa E.S., Lucas B. & Calos R.S.(2018). Solid-state fermentation for the production of mushrooms. Current Developments in Biotechnology and Bioengineering. pp. 285-318.

    Luyen N.T., Ve V.L., Nguyen B.T.T., Nguyen H.T.T., Tran A.D., Ngo N.X. (2023). Optimization of mycelial growth and cultivation of wild Ganoderma sinense. Journal of Biotechnology, Computational Biology and Bionanotechnology. 104(1): 65-74.

    Ma Y., Guan C.Y. & Meng X.J. (2014) Biological characteristics for mycelial growth of Agaricus bisporus. Applied Mechanics and Materials. 508: 297-302.

    Mallikarjuna S.E., Ranjini A., Haware D.J., Vijayalakshmi M.R., Shashirekha M.N. & Rajarathnam S. (2013). Mineral Composition of Four Edible Mushrooms. Journal of Chemistry. pp. 1-5.

    Manu-Tawiah W. & Martin A.M. (1988). Nitrogen sources and the growth response of Pleurotus ostreatus mushroom mycelium. Canadian Institute of Food Science and Technology Journal. 21: 194-199.

    Mikeš V., Zofall M., Chytil M., Fulneček J. & Scháně L. (1994). Ammonia-assimilating enzymes in the basidiomycete fungus Pleurotus ostreatus. Microbiology. 140: 977-982.

    Miles P.G. & Chang S.T. (1997). Mushroom biology: Concise basics and current developments. In: Miles, PG, editor. Mushroom Biology: Concise Basics and Current Developments. Singapore: World Scientific Publishing Company. 41.

    Min G.J., Park H.S., Lee E.J. & Lee C.J. (2020) Culture characteristics and optimal conditions for mycelial growth of Calocybe indica. The Korean Journal of Mycology. 48(3): 273-284

    Nguyễn Văn Giang, Nguyễn Thị Bích Thuỳ, Vũ Thị Khánh Linh, Nguyễn Duy Trình & Trần Thu Hà (2021). Ảnh hưởng của điều kiện nuôi cấy đến sinh trưởng hệ sợi của nấm Phellinus linteus. Tạp chí Khoa học Nông nghiệp Việt Nam. 19(2):39-43.

    Nguyen B.T.T., Le V.V, Nguyen H.T.T., Nguyen L.T., Tran T.T.Y. & Ngo N.X. (2021). Nutritional requirements for the enhanced mycelial growth and yield performance of Trametes versicolor. Journal of Applied Biology and Biotechnology. 9(1): 1-7.

    Nguyen B.T.T., Nghien X.N., Ve V.L., Luyen N.T.,Anh D.T & Lam H.T.N (2018). Identification of optimal culture conditions for mycelial growth and cultivation of monkey head mushrooms (Hericium erinaceus(Bull.: Fr.). Vietnam Journal of Agricultural Sciences. 1(2): 117-126.

    Nguyen B.T.T.,Ngo N.X.,Le V.V.,Nguyen L.T.,Kana R.&Nguyen H.D. (2019). Optimal culture conditions for mycelial growth and fruiting body formation of Ling Zhimushroom Ganoderma lucidumstrain GA3. Journal of Science Technology and Engineering. 61(1): 62-67.

    Nguyen N.P.D & Khanh T.D. (2017). Impacts of Ecological Factors on the Distribution of Amauroderma murrill genus in central highlands of Vietnam. Journal of Scientific and Engineering Research. 4(9): 238-243.

    Ohta A. (1994). Production of fruit-bodies of a mycorrhizal fungus, Lyophyllum shimeji, in pure culture. Mycoscience. 35: 147-151.

    Peng S.H.T., Yap C.K., Ren P.F. & Chai E.W. (2019). Effects of environment and nutritional conditions on mycelial growth of Ganoderma boninense. International Journal of Oil Palm. 2(3): 95-107.

    Shim S.M., Oh Y.H., Lee K.R., Kim S.H., Im K.H., Kim J.W., Lee U.Y., Shim J.O., Shim M.W., Lee M.W., Ro H.S., Lee H.S. & Lee T.S. (2005) The characteristics of cultural conditions for the mycelial growth of Macrolepiota procera. Mycobiology. 33(1): 15-18.

    Ta K., Vyu B. & As S. (2021) Review of the basic cultivation conditions influence on the growth of basidiomycetes. Current Research in Environmental & Applied Mycology. 11(1): 494-531.

    Trịnh Tam Kiệt (2012). Nấm lớn ở Việt Nam (Tập 2). Nhà xuất bản Khoa học Tự nhiên vàCông nghệ, Hà Nội. 412tr.

    Wang Q., Wang Y.G., Ma Q.Y., Huang S.Z., Kong F.D., Zhou L.M., Dai H.F. & Zhao Y.X. (2016). Chemical constituents from the fruiting bodies of Amauroderma subresinosum. Journal of Asian natural products research. 18(11): 1030-1035.

    ZhengC.W., CheungT.M.Y. & LeungG.P.H. (2022). A review of the phytochemical and pharmacological properties of Amauroderma rugosum. The Kaohsiung Journal of Medical Sciences. 38(6): 509-516.