Mycelial GrowthCharacteristics andFormationandDevelopmentofFruiting BodiesofNaturalLingzhi (Ganoderma orbiforme) FM6 Collected in Pu Mat National Park

Received: 03-10-2023

Accepted: 25-12-2023

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Issue: Vol. 21 No. 12 (2023)

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NÔNG HỌC

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Luyen, N., Trang, N., Nghien, N., & Canh, N. (2024). Mycelial GrowthCharacteristics andFormationandDevelopmentofFruiting BodiesofNaturalLingzhi (Ganoderma orbiforme) FM6 Collected in Pu Mat National Park. Vietnam Journal of Agricultural Sciences, 21(12), 1622–1634. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1233

Mycelial GrowthCharacteristics andFormationandDevelopmentofFruiting BodiesofNaturalLingzhi (Ganoderma orbiforme) FM6 Collected in Pu Mat National Park

Nguyen Thi Luyen (*) 1 , Nguyen Thi Huyen Trang 2 , Ngo Xuan Nghien 2, 1 , Nguyen Xuan Canh 1

  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 2 Viện Nghiên cứu và Phát triển Nấm ăn, Nấm dược liệu, Học viện Nông nghiệp Việt Nam

    • Keywords

    Lingzhi, fruitbody, mycelial, substrate, PuMat, Vietnam

    Abstract


    FM6lingzhistrainwasdiscoveredgrowingnaturallyinPuMatNationalPark, ConCuongDistrict,NgheAnProvine.This study aims to use the ITS4/ITS5 primer pair to amplify the ITS region of the new collected mushroom strain to identify this strainandinitiallyinvestigatethegrowthcharacteristicsofthemyceliaondifferent carbonandnitrogensources.Inaddition,theeffectofwheatbranratioaddedtothe culturesubstrateonthemycelialgrowthandtheformationoffruitingbodiesofthisstrain was evaluated.The results ofsequencingandrunningBLASTonNCBIshowedthatFM6strainwasmorethan99% similartothe speciesGanodermaorbiforme.Fructose(15g/l) andNH4Cl(2g/l)werethemost suitablecarbonandnitrogensources,respectively,forthegrowth of lingzhistrainFM6.Substrate supplementedwith 10%wheatbranperformedthehighestBE,reaching 8.84%

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