Evaluating Properties of Mycelial Growth and Effect of the Wheat Bran Ratio on Formation and Fruiting Body Development of Golden Lingzhi Mushroom Strain(Tomophagus cattenensis)

Received: 15-08-2023

Accepted: 25-12-2023

DOI:

Views

4

Downloads

1

Issue: Vol. 21 No. 12 (2023)

Section:

NÔNG HỌC

How to Cite:

Trang, N., Nghien, N., Anh, T., Luyen, N., & Thuy, N. (2024). Evaluating Properties of Mycelial Growth and Effect of the Wheat Bran Ratio on Formation and Fruiting Body Development of Golden Lingzhi Mushroom Strain(Tomophagus cattenensis). Vietnam Journal of Agricultural Sciences, 21(12), 1581–1591. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1229

Evaluating Properties of Mycelial Growth and Effect of the Wheat Bran Ratio on Formation and Fruiting Body Development of Golden Lingzhi Mushroom Strain(Tomophagus cattenensis)

Nguyen Thi Huyen Trang (*) 1 , Ngo Xuan Nghien 2 , Tran Dong Anh 1 , Nguyen Thi Luyen 1 , Nguyen Thi Bich Thuy 3, 2

  • 1 Viện Nghiên cứu Phát triển Nấm ăn, Nấm dược liệu, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 3 Viện Nghiên cứu và Phát triển Nấm ăn, Nấm dược liệu, Học viện Nông nghiệp Việt Nam

    • Keywords

    Tomophagus cattienensis, cultivation, mineral salt sources, temperature, wheat bran ratios

    Abstract


    The golden lingzhi strain Toc1 (Tomophagus cattienensis)is a new strain that was discovered in the Cat Tien National Park in Đong Nai provine and it was identified to be a precious mushroom species. In this study, mycelial of strain Toc1 were incubated in 4 temperature levels (20 ± 1C, 25 ± 1C, 30 ± 1Cand 35 ± 1C); culture medium supplemented 5 different carbon sources (glucose, fructose, saccharose, maltose and lactose), 5 different nitrogen sources (yeast extract, peptone, ammonium clorua, ammonium nitrate and ammonium sulfate), 6 mineral salt sources (kali cloride, kali nitrate, natri nitrate, dikali phosphat, kali dihydrophosphat and magie sulfate). Beside that, mycelial of this strain were cultivated in 4 substrates added different wheat bran ratio (0%, 5%, 10% and15%). This study aims to figue out the optimal temperature, the suitable carbon, nitrogen and mineral salt source for mycelial growth of golden lingzhi mushroom strain Toc1 and also evaluating effect of the wheat bran ratio, which was supplemented on substrate, on formation and fruiting body development of this strain. The results showed that the optimal temperature for mycelial growth is 25C±1. Glucose and yeast extract were considered the most suitable carbon and nitrogen sources for mycelial growth. This strain grew very well on culture supplemented K2HPO4. The substrate containing 89% sawdust, 5% corn powder, 1% CaCO3and 5% wheat bran performed the highest BE, reached 15,97%.

    References

    Andrew L. Loyd, Eric R. Linder, Matthew E. Smith, Robert A. Blanchette & Jason A. Smith (2019). Cultural characterization and chlamydospore function of the Ganodermataceaepresent in the eastern United States. Mycologia. 111(1): 1-12. doi: 10.1080/ 002 755 14. 2018.1543509.

    Bui Thi Thu Hien, Le Thi Phuong Hoa, Le Xuan Tham & Dang Ngoc Quang (2013). Cattienoids A-C, three novel steroids from the mushroom Tomophagus cattienensis. Fitoterapia. 9: 125-127. https://doi.org/10.1016/j.fitote.2013.08.020

    Chang S. & Miles G.P. (2004). Mushroom: Cultivation, nutritional value, medicinal effects and environmental impact. Boca Raton, FL: CRC press. p. 436

    Đinh Xuân Linh, Thân Đức Nhã & Nguyễn Thị Sơn (2007). Kỹ thuận trồng, chế biến nấm ăn, nấm dược liệu. Nhà xuất bản Nông nghiệp.

    Elhami B., Ansari N.A. & Dehcordie F.S. (2008). Effect of substrate type, different level of nitrogen and maganese on growth and development of oyster mushroom (Pleurotus florida). Dyn. Biochem. Process Biotechnol. Mol. Biol. 2(1): 34-37.

    Fanadzo M., Zireva D.T., Dube E. & Mashingaidze A.B. (2010). Evalution of various substrates and supplements for biological efficiency of Pleurotus sajor-cajuand Pleurotus ostreatus. African Journal of Biotechnology. 9(19): 2756-2761

    George G. Songulashvili, Vladimir Elisashvili, Solomon P. Wasser, Yitzhak Hadar & Eviatar Nevo (2008). Effect of the cacbon source and inoculum preparation method on laccase and manganese peroxidase production in submerged cultivation by the medicinal mushroom Ganoderma lucidum(W. Curt.:Fr.) P. Karst. (Aphyllophoromycetideae). International Journal of Medicinal mushrooms. 10(1): 79-86.

    Ian Fletcher, Aisha Freer, Ash Ahmed & Pauline Fitzgerald. (2019). Effect of temperature and growth media on mycelium growth of Pleurotus ostreatusand Ganoderma Lucidumstrains. Cohesive Journal of Microbiology and infectious disease.

    Itoo Z.A. & Reshi Z.A. (2014). Effect of different nitrogen and carbon sources and concentrations on the mycelial growth of ectomycorrhizal fungi under in-vitroconditions. Scandinavian Journal of Forest Research. 29(7): 619-628.

    Jeong Y.T., Yang B.K., Jeong S.C., Gu Y.A., Kim G.N., Jeong H. & Song C.H. (2005). “Optimum conditions for the mycelial growth and exo-biopolymer production by a submerged culture of Elfvingia applanata”. Korean J Mycol News Letter.17: 97.

    Le Xuan Tham, Quoc Hung Nguyen Le, Ngoc Duong Pham, Van Hop Duong, Dentinger B.T.M. & Moncalvo J.M. (2012). Tomophagus cattienensis sp. nov., a new Ganodermataceae species from Vietnam: Evidence from morphology and ITS DNA barcodes. Mycological Progress. 11(3): 777.

    Mallikarjuna S.E, Ranjini A, Haware D.J, Vijaylakshmi M.R, Shashirekha M.N. & Rajarathnam S. (2013). Mineral composition of four edible mushrooms. Journal of chemistry. pp. 1-5

    Miles P.G. & Chang S.T. (1997). Mushroom biology: Concise basics and current developments. In P.G. Miles (Ed.), Mushroom biology: Concise basics and current developments. World scientìic publishing company.

    Murrill WA. (1905). Tomophagusfor Dendrophagus. Torreya. 5: 197

    Narain R., Sahu R., Kumar K.S., Garg S.K., Singh C.S. & Kanaujia R.S. (2008). Influence of different nitrogen rich supplements during cultivation of Pleurotus floridaon maize cobs substrate. Environmentalist. 29: 1-7.

    Nguyen B.T.T., Van Le V., Nguyen H.T.T., Nguyen L.T., Tran T.T.T. & Ngo N.X. (2021b). Nutritional requirements for the enhanced mycelial growth and yield performance of Trametes versicolor. J. Appl. Biol. Biotechnol. 9: 1-7. doi.org/10.7324/JABB. 2021.9101.

    Nguyễn Thị Huyền Trang, Lê Văn Vẻ, Phan Thị Huyền Trang, Nguyễn Thị Luyện, Ngô Xuân Nghiễn & Nguyễn Thị Bích Thùy (2022). Sinh trưởng của hệ sợi và phát triển của quả thể nấm sò vàng (Pleurotus cintrinopileatus) trên một số môi trường dinh dưỡng khác nhau. Tạp chí khoa học nông nghiệp Việt Nam. 20(5): 642-651.

    Singer B., Moonmoon M., Jahan N., Khan A., Uddin N., Hossain K., Tania M. & Ahmed S. (2011). Effects of different levels of wheat bran, rice bran, and maize powder supplementation with sawdust on the production of shiitake mushroom. Saudi Journal of Biological sciences. 18(4): 323-328.

    Trịnh Tam Kiệt (2012). Nấm lớn ở Việt Nam (Tập 2). Nhà xuất bản Khoa học tự nhiên và Công nghệ.

    Woo-Sik Jo, Young-Hyun Rew, Sung-Guk Choi, Geon-Sik Seo, Jae-Mo Sung & Jae-Youl Uhm. (2006). The culture conditions for the mycelial growth of Phellinus spp.Mycrobiology. 34(4): 200-205.

    Woo-Sik Jo, Yun-Ju Cho, Doo-Huyn Cho, So-Deuk Park, Young-Bok Yoo & Soon-Ja Seok. (2009). Culture conditions for the mycelial growth of Ganoderma applanatum. Mycobiology. 37(2): 94-102

    Zadrazil F. (1976). The ecology and industrial production of Pleurotus ostreatus, Pleurotus florida, Pleurotus cornucopiae, and Pleurotus eryngii. Mushroom Sci. 9: 621-652.