In VitroEffects of Minerals on Sporulation and Heat Resistance of BacillussubtilisCM3.1

Received: 29-03-2023

Accepted: 05-10-2023

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Issue: Vol. 21 No. 10 (2023)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Ngan, P., Hai, V., & Giang, H. (2024). In VitroEffects of Minerals on Sporulation and Heat Resistance of BacillussubtilisCM3.1. Vietnam Journal of Agricultural Sciences, 21(10), 1249–1255. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1195

In VitroEffects of Minerals on Sporulation and Heat Resistance of BacillussubtilisCM3.1

Pham Thi Tuyet Ngan (*) 1 , Vu Hung Hai 1 , Huynh Truong Giang 1

  • 1 Trường Thủy sản, Đại học Cần Thơ

    • Keywords

    Bacillus, sporulation, heat resistance, minerals, spore cell density

    Abstract


    This study sought to evaluate the in vitro effects of minerals, including calcium (Ca2+),potassium (K⁺), manganese (Mn2+),magnesium (Mg2+) and iron(Fe2+) on sporulation efficiency and heat resistance of BacillussubtilisCM3.1. Sporulation of Bacillus cells was induced in sporulation medium supplemented with various concentrations of the minerals. 7 days after inoculation, Ca2+(1mm), Mg2+(3mm), Mn2+(1mm) and Fe2+(1mm) as single supplements into Nutrient Broth (NB) significantly increased spore cell density of BacillussubtilisCM3.1, except for K+(10mm). Furthermore, the spore cell density and viability of Bacillus were detectable in sporulation media such as m-NB, SSM and m-SM, significantly higher compared to those in NB (P <0.05) after heating periods, especially from 30 to 40 minutes of exposure. Our results could provide better understanding of the importance of minerals supplemented into culture medium in improving the density, heat resistance of Bacillus spore, and thus enhancingthe stability of probiotic Bacillus subtilis CM3.1 during manufacturing process and storage time.

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