Determining the Prevalence of Porcine Adenovirus (PAdV) in PigsRaisedin some Provinces in the North of Vietnam

Received: 31-12-2022

Accepted: 27-03-2023

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Issue: Vol. 21 No. 3 (2023)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Truong, L., Le, H., Anh, D., Giap, N., Ngan, M., Hieu, V., … Phan, L. (2024). Determining the Prevalence of Porcine Adenovirus (PAdV) in PigsRaisedin some Provinces in the North of Vietnam. Vietnam Journal of Agricultural Sciences, 21(3), 289–296. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1115

Determining the Prevalence of Porcine Adenovirus (PAdV) in PigsRaisedin some Provinces in the North of Vietnam

Le Van Truong (*) 1 , Huynh Thi My Le 1 , Dang Huu Anh 1 , Nguyen Van Giap 1 , Mai Thi Ngan 1 , Vo Van Hieu 1 , Trinh Huong Ly 1 , Le Van Phan 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Porcine adenovirus, nested PCR, piglets, prevalence

    Abstract


    Porcine adenovirus (PAdV) is the causative agent of diarrhea in piglets aged one to four weeks. Among the three species of adenoviruses (AdVs) that cause disease in pigs, A, B, and C, six serotypes have been identified through neutralization methods. Our study aimed to determine the prevalence of PAdV in pigs raised in Hanoi and its surrounding areas. One hundred and forty fecal samples were collected from piglets exhibiting diarrhea symptoms and subjected to DNA extraction. Nested PCR was then used to detect PAdV in the samples. Positive PCR samples were purified and sequenced. The results showed that the nested PCR method was effective in diagnosing PAdV causing diarrhea in pigs, with 43.57% (61/140) of the samples testing positive. Ten out of fourteen (71.43%) farms showed a prevalence of PAdV. The sequencing results of the PAdV hexon gene segment of the positive samples identified six strains of PAdV circulating in Vietnam, including PAdV-8, PAdV-25, PAdV-26, PAdV-28, PAdV-33, and PAdV-34. The molecular epidemiological analysis of PAdV also revealed that all six strains isolated in Vietnam belonged to PAdV serotype 3, a species of Adenovirus A, genus Mastadenovirus.

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