Favorable Culture Conditions for Mycelial Growth of Mutated Strain DTL1,25of Lingzhi Mushroom (Ganoderma lucidum)

Received: 27-09-2022

Accepted: 20-12-2022

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Issue: Vol. 20 No. 12 (2022)

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KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Giang, N., Thu, P., Hien, P., & Hoi, P. (2024). Favorable Culture Conditions for Mycelial Growth of Mutated Strain DTL1,25of Lingzhi Mushroom (Ganoderma lucidum). Vietnam Journal of Agricultural Sciences, 20(12), 1649–1658. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1081

Favorable Culture Conditions for Mycelial Growth of Mutated Strain DTL1,25of Lingzhi Mushroom (Ganoderma lucidum)

Nguyen Thi Giang (*) 1 , Pham Thi Thu 1 , Pham Bich Hien 2 , Pham Xuan Hoi 1

  • 1 Viện Di truyền Nông nghiệp
  • 2 Viện Khoa học Nông nghiệp Việt Nam

    • Keywords

    Ling Zhi (Ganoderma lucidum), mycelial growth, nutrient environment and culture conditions

    Abstract


    The objective in this study was to determine the optimal nutrient environment and culture conditions for mycelial growth of mutated strain DTL1,25 of Lingzhi (Ganoderma lucidum). Each treatment was randommized complete block design andreplicated three times. The results of the experiment indicated that in the first stage of propagation, the optimal medium for mycelial growth was the background medium from the extract of 200g barley seeds supplemented with 2% glucose, 0.1% malt extract and 2% agar with medium pH of 6 and temperature at 26 ± 1C. In the second stage of propagation, mycelium growth of the mutant strain DTL1,25 was improved on environment consisting of 99% rice grain + 1% CaCO3with autoclaving mode at 121C for 120 minutes. At the third stage, the optimal composition included 99% rice grain + 1% CaCO3with autoclaving mode at 121C for 150 minutes.

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