Effects of Culture Conditons on IAA Synthetic Ability of Endophytic Bacterial Strains HY9 and TT3

Received: 24-06-2022

Accepted: 22-11-2022

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Issue: Vol. 20 No. 12 (2022)

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KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Hien, P., Dao, T., Trang, T., Lieu, H., & Giang, N. (2024). Effects of Culture Conditons on IAA Synthetic Ability of Endophytic Bacterial Strains HY9 and TT3. Vietnam Journal of Agricultural Sciences, 20(12), 1640–1648. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1080

Effects of Culture Conditons on IAA Synthetic Ability of Endophytic Bacterial Strains HY9 and TT3

Pham Hong Hien (*) 1 , Tran Thi Dao 2 , Ta Ha Trang 2 , Hoang Thi Lieu 2 , Nguyen Van Giang 2

  • 1 Viện Khoa học Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Lonicera japonica, Endophytes, IAA, Klebsiella spp

    Abstract


    Endophytic bacteria living in the plant tissues support plant growth by synthesizing IAA and siderophore, inorganic soluble phosphate, and increasing disease resistance of the host plants. This study aimed to evaluate the effects of carbon and nitrogen sources, pH, temperature, and time of incubation on the concentration of IAA synthesized by two endophytic bacterial strains, HY9 and TT3, isolated from Lonicera japonica. Under various suitable culture conditions, maximum IAA of HY9 and TT3 strains was produced at pH=7 (42.9 µg/ml and 49.07 µg/ml, respectively), 35C (53.49 µg/ml and 56.73 µg/ml), and 4 days of incubation (51.47 µg/ml and 53.76 µg/ml). The suitable carbon and nitrogen sources were saccharose and NH4Cl. Comparing the nucleotide sequence of 16S rRNA of TT3 strain with nucleotide sequences of 16S rRNA of other bacterial strains on GenBank revealedthat TT3 strain had a very closed relationship with Klebsiella variicola strain 1, thus,strain TT3 was named Klebsiella variicola TT3.

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