Genetic Diversity and Population Structure of Passion Fruit (Passiflora edulis Sim.) Accessions Revealed by SRAP and ISSR Markers

Received: 14-10-2021

Accepted: 01-03-2022

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Issue: Vol. 20 No. 4 (2022)

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NÔNG HỌC

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Trung, T., Viet, N., & Dang, B. (2024). Genetic Diversity and Population Structure of Passion Fruit (Passiflora edulis Sim.) Accessions Revealed by SRAP and ISSR Markers. Vietnam Journal of Agricultural Sciences, 20(4), 425–435. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/984

Genetic Diversity and Population Structure of Passion Fruit (Passiflora edulis Sim.) Accessions Revealed by SRAP and ISSR Markers

Tran Duc Trung (*) 1 , Nguyen Van Viet 2 , Bui Quang Dang 1

  • 1 Viện Khoa học Nông nghiệp Việt Nam
  • 2 Viện Nghiên cứu và Phát triển Nông nghiệp Nafoods

    • Keywords

    Passiflora, genetic diversity, population structure, ISSR, SRAP

    Abstract


    Identification of potential germplasms is the crucial step for selection and breeding program of passion fruit (Passiflora edulisSims.). In this study, genetic diversity and population structure of 31 exotic passion fruit accessions (including wild relative P. incarnata accessions) were assessed using sequence-related amplified polymorphism (SRAP) and inter simple sequence repeat (ISSR) markers. By primary screening for marker specificity and polymorphism, 8/15 SRAP markers and 10/18 ISSR were selected for passion fruit genotyping. The heterozygosity index H(0.43), polymorphic information content PIC(0.35), and discriminating power D(0.8) were recorded at a high level implying the significant genetic diversity of the analyzed passion fruit accessions. The Neighbor-Joining phylogenetic tree in combination with population structure model revealed by STRUCTURE discriminated and clustered the studied accessions into five groups which showed high accordance with the fruit color and the origin of the accessions. The analysis of melecular variance (AMOVA) also indicated the high rate of intraspecific and possibility interspecific hybridization among Passifloraspecies. These results provide essential genetic background facilitating efficient conservation and utilization of exotic passion fruit accessions in Vietnam.

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