Received: 15-06-2021
Accepted: 02-07-2021
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Factors Affecting the Carotenoid Production by Red Yeast RhodotorulaStrains used to Produce Carotene-rich Feed for Layers
,
Le Viet Phuong
1
,
Bui Quang Tuan
1
,
Bui Huy Doanh
1
Keywords
Red yeast, Rhodotorula, carotenoids
Abstract
The objectives of this work were to select carotenoid-producing red yeast strains and assess the impact of various parameters such as temperature, pH, C and N sources on dry biomass and their carotenoid production. A total of 8 Rhodotorulastrains, viz. SR1, SR2, SR3, SR4, SR5, SR6, SR7, SR8 were inoculated on Hansen broth at 30C/72h. The highest carotenoid producing yeast strains SR6 and SR7 (0.76 and 0.81 mg/gDM) were selected to further study the effect of inoculation percentages, temperature, pH, as well as C and N sources on growth and carotenoid production. The results showed that the two selected red yeast strains grew fast and produced the highest carotenoid when cultrured on liquid Hansen medium with 2% innoculation rate at 30C and pH 5.5. The optimum sources of N and Cfor red yeast culture medium were petptone and glucose, respectively. Corn meal and rice flour were chosen as the best substrates for solid fermentation.
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