Molecular and Biological Characteristics of African Swine Fever Virus Isolated in Some Northern Provinces of Vietnam

Received: 25-06-2020

Accepted: 03-09-2020

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Issue: Vol. 18 No. 10 (2020)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Ngoc, T., Tam, N., Huyen, N., Dang, V., & Phan, L. (2024). Molecular and Biological Characteristics of African Swine Fever Virus Isolated in Some Northern Provinces of Vietnam. Vietnam Journal of Agricultural Sciences, 18(10), 803–811. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/718

Molecular and Biological Characteristics of African Swine Fever Virus Isolated in Some Northern Provinces of Vietnam

Trinh Thi Bich Ngoc (*) 1 , Nguyen Van Tam 1 , Nguyen Thi Thu Huyen 1 , Vu Xuan Dang 1 , Le Van Phan 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    African swine fever, virus isolation, phylogeny

    Abstract


    African swine fever (ASF) is a highly infectious disease in the domestic and wild pig populations causing tremendous damage to the global swine industry. ASF was first reported in Vietnam on February 1st, 2019 and the disease has spread to 63 provinces in just 7 months. This study aims to investigate the molecular and biological characteristics of ASF viruses isolated from tissue samples of ASF-infected pigs collecting from the northern provinces of Vietnam. The results of virus isolation showed that five ASFV strains had been successfully isolated on PAM (Porcine Alveolar Macrophage) cells, virus titer ranging from 106-107,5 HAD50/ml. The results of the growth curve of the virus on PAM cells disclosed that, with the multiplicity of infection (MOI) = 1, the highest achieved virus titer was 108.160.21HAD50/ml after 96 hours post-infection. The sequencing results of the B646L (p72) gene showed that all present ASFV strains shared 100% nucleotide and amino acid sequence identity with each other. Phylogenetic analysis revealed that all isolated strains belonged to genotype II.

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