Received: 22-05-2020
Accepted: 21-06-2020
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Optimization of Porcine Oocyte in VitroMaturation Medium for Application in Vietnam
,
Hoang Thi Kim Chi
1
,
Nguyen Thi Ngoc Anh
1
,
Nguyen Thi Hong Nhung
1
,
Kazuhiro Kikuchi
2
,
Takeshige Otoi
3
,
Nguyen Thi Thu Trang
4
,
Su Thanh Long
1
Keywords
Porcine oocytes, maturation, hormones
Abstract
The present study was conducted to evaluate the maturation rate of porcine oocytes matured in POM or TCM199 medium; in maturation, medium made from Sigma pure water or MQW and effects of hormone supplementation in the first 22 hours or during IVM. Maturation rate of porcine oocyte matured in POM (73.45 ± 2.61%) medium was significantly higher than that of TCM199 medium (58.84 ± 2.49). There was no significant difference in the maturation rate of porcin oocyte matured in POM medium made from Sigma pure water (87.16 ± 4.74%) or MQW (83.20 ± 6.93%). The supplementation of hormone in 22 hours could help to improve the maturation rate (90.88 ± 2.33%) compared to that of 46 hours (84.93 ± 2.78%). MQW can be used to prepared porcine oocyte matured medium. Supplementation of hormones during IVM1 has potential to improve the maturation rate of porcine oocytes.
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