Received: 20-11-2019
Accepted: 16-06-2020
DOI:
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Study on Expression of Chicken Interleukin 2 Recombinant Protein and its Modulate Cytokines Production in Macrophage Cell Line
,
Tran Thi Thanh Ha
1
,
Nguyen Thi Huyen
1
,
Chu Thi Nhu
1
,
Nguyen Thi Chinh
1
,
Hoang Van Tuan
1
,
Ly Duc Viet
1
,
Dang Vu Hoang
1
,
Yeong Ho Hong
2
Keywords
Escherichia coli, Interleukin-2, chicken, cytokines, recombinant protein
Abstract
Interleukin-2 is a multifunctional cytokine, which modulates the proliferation and differentiation of various types of cells and induces cytokines expression. In this study, chicken IL-2 will be cloned, expressed and its functional characterization was evaluated in Macrophage (HD11) cell line. Genes coding for IL-2 (AF000631 from GenBank) linked with Trx-Tag thioredoxin leader and restriction sequences of EcoRI (5’end), HindIII (3’end) were cloned and then ligated into a cloning véc tơvector pCR2.1-TA. Chicken IL-2 gene was incorporated into pET32a(+) at restriction sites for the expression of the IL-2 gene in E. coliBL21. Chicken IL-2 recombinant protein produced in E. colisystem showed that it did not produce cytotoxicity and enhanced the proliferation of macrophage (HD11) cell line and induced IFNγ, IL-1β và and IL-6 mRNA expression. These results showed that recombinant chicken IL-2 protein production in E. colisystem was possessed natural biological properties and was recognized by specific antibodies against IL-2.
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