Isolation and Identification of Avibacteriumparagallinarumfrom Chickens in North of Vietnam

Received: 19-07-2019

Accepted: 22-10-2019

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Issue: Vol. 17 No. 8 (2019)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Hung, L., Giang, N., & Son, T. (2024). Isolation and Identification of Avibacteriumparagallinarumfrom Chickens in North of Vietnam. Vietnam Journal of Agricultural Sciences, 17(8), 622–629. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/587

Isolation and Identification of Avibacteriumparagallinarumfrom Chickens in North of Vietnam

Le Van Hung (*) 1 , Nguyen Thi Giang 1 , Tran Danh Son 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Av. paragallinarum, isolation, antibiotic resistance

    Abstract


    Infectious coryza (IC) is an infectious upper respiratory disease affecting chickens and it is caused by Haemophilus paragallinarum (or Avibacterium paragallinarum). The symptoms of IC are facial swelling, malodorous nasal discharge and lacrimation. This study aimed to isolate, identify the Av. paragallinarumof snot in chickens and to determine the sensitivity and resistance to several antibiotics. Ten isolates out of thirteen suspected isolates (76,92%) were Av. paragallinarumby the observation of the morphology of the suspected colony, Gram staining, and biochemical tests (catalase test, oxidase test, urease test, indole test; carbohydrate fermentation such as maltose, lactose, mannitol and sorbitol; motility). The growth of isolates from chickens was not depended on the nicotinamide adenine dinucleotide (NAD). Sensitivity test was done using the ten identified Av. paragallinarumisolates that were cultured on Mueller-Hinton agar and added with antibiotic discs, then incubated in 5% CO2at 37C for 24-48h; results showed that they were 100% sensitive to amoxicillin, ampicillin; 60% sensitive to neomycin, penicillin and 40% sensitive to streptomycin; 100% resistant toward erythromycin, gentamycin, tetracyclin, kanamycin and enrofloxacin.

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