Received: 27-08-2018
Accepted: 10-05-2019
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Molecular Characterization of Canine Parvovirus Type 2Isolated from Dogs in Hanoi
Keywords
Canine Parvovirus, dogs, molecular charaterization, phylogenetic tree
Abstract
Canine Parvovirus type 2 (CPV-2) has caused a highly contagious and fatal disease characterized by vomiting and hemorrhagic gastroenteritis in dogs of all age.In this study, four diarrheal stool samples collected from Parvovirus-infected dogs were used for VP2 gene sequencing. The results of VP2 gene sequencing and analysis of present CPV strains showed high nucleotide (nt) and amino acid (aa) identities when compared with those of other reference worldwide strains, sharing 98.4 - 100% nt and 97.7 - 100% aa. The results of the phylogenetic tree showed that the Parvovirus type 2 in this study was genetically classified into two distinct groups of Feline Panleukopenia Virus (FPV) and CPV. Three strains VNUA-CP31, VNUA-CP32 and VNUA-CP35 belonged to the branch of FPV and close to the FPV in China. Parvovirus type 2 VNUA-CP17 belongsto the branch of CPV and is close to Parvovirus vaccines of the Pfizer and South Korea.
References
Appel MJG, Cooper BJ, Greisen H, et al. (1978). Status report: canine viralenteritis. J Am Vet Med Assoc. 173: 1516-1518.
ChinchkarS.R., Subramanian B.M., Rao N.H., Rangarajan P.N., Thiagarajan D. & SrinivasanV.A. (2006). Analysis of VP2 gene sequences of canine parvovirus isolates in India. Arch Virol.151:1881-1887.
Decaro N., Desario C., Addie D.D., Martella V., Vieira M.J., Elia G. & Buonavoglia C. (2007). The study molecular epidemiology of canine parvovirus.Europe. Emerg Infect Dis. 13, 1222.
DecaroN. &BuonavogliaC.(2012). Canine Parvovirus- A review of epidemiologicaland diagnostic aspects with emphasis on type 2c. Vet Microbiol. pp.155, 1-12.
Decaro N., Desario C., Amorisco F., Losurdo M.,Colaianni M. & Buonavoglia C.(2011). Canine parvovirus type 2c infection in a kitten associated with intracranial abscess and convulsions. J Feline Med Surg.13:231-236.
Hueffer K., Parker J.S., Weichert W.S., Geisel R.E., Sgro J.Y. & Parrish C.R. (2003). The natural host range shift and subsequent evolution of canine parvovirus resulted from virus-specific binding to the canine transferrin receptor.J Virol. 77: 1718-1726
Johnson R.H., Margolis G. &Kilham L.(1967).Identity of feline ataxia virus withfeline panleukopenia virus. Nature.214(5084):175-177.
KumarS., StecherG.&TamuraK.(2016). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol.33:1870-1874.
Lin C.N., ChienC.H., ChiouM.T., ChuehL.L., HungM.Y. & HsuH.S. (2014). Genetic characterization of type 2a canine parvoviruses from Taiwan reveals the emergence of an Ile324 mutation in VP2. Virol J.11: 39.
Mochizuki M., Horiuchi M., Hiragi H., San Gabriel M.C., Yasuda N. & Uno T.(1996).Isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia.JournalClin Microbiol.34(9):2101-2105.
Martella V., Decaro N., Buonavoglia C.(2006). Evolution of CPV-2 andimplication for antigenic/genetic characterization. Virus Genes. 33(1): 11-13.
Mira F., Purpari G., Lorusso E., Di Bella S., Gucciardi F., Desario C., Macaluso G., Decaro N. & Guercio A. (2018). Introduction of Asian canine parvovirus (CPV) in Europe through dog importation. Transbound Emerg Dis.65(1):16-21.
Nakamura M, Tohya Y, Miyazawa T, Mochizuki M, Phung HT, Nguyen NH. (2004). A novel antigenic variant of canine parvovirus from a Vietnamese dog. Arch Virol.149:2261-2269.
Truyen U. & Parrish C.R.(1992). Canine and Feline host ranges of canine Parvovirus and Feline Panleukopenia virus: distinct host cell tropisms of each virus in vitroand in vivo. J Virol. pp. 5399-5408.
Wu J., Gao XT., Hou SH., Guo XY., Yang XS., Yuan WF., Xin T., Zhu HF., Jia H. (2015). Molecular epidemiological and phylogenetic analyses of canine parvovirus in domestic dogs and cats in Beijing, 2010-2013. J Vet Med Sci.77 (10): 1305-1310.
Zhong Z., Liang L., Zhao J., Xu X., Cao X., Liu X. & Peng G. (2014). First isolation of new canine parvovirus 2a from Tibetan Mastiff and global analysis of the full length VP2 gene of canine parvoviruses 2 in China. Int J Mol Sci.15: 12166-12187.