Production and Characterization of Lignin Peroxidase from White Rot Fungal Strain Tl4

Received: 29-12-2016

Accepted: 12-11-2018

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Issue: Vol. 16 No. 8 (2018)

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KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Thuy, T., Trung, N., Hai, T., & Anh, N. (2024). Production and Characterization of Lignin Peroxidase from White Rot Fungal Strain Tl4. Vietnam Journal of Agricultural Sciences, 16(8), 763–771. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/493

Production and Characterization of Lignin Peroxidase from White Rot Fungal Strain Tl4

Trinh Thi Thu Thuy (*) 1 , Nguyen Quoc Trung 1 , Tong Van Hai 1 , Nguyen Hoang Anh 2

  • 1 Khoa Công nghệ Sinh học, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ Thực phẩm, Học viện Nông nghiệp Việt Nam

    • Keywords

    Lignin peroxidase (LiP), white rot fungus, characterization, thermal stability, pH stability

    Abstract


    Lignin is a component of plant cell wall. The degradation of lignin remains a challenge to date due to its stability. Lignin peroxidase (LiP) from white rot fungi was known as a highly effective oxidase enzyme for lignin degradation. In this study, extracellular LiP obtained from strain TL4 was precipitated by (NH4)2SO4and ethanol 96% for obtaining technical enzyme and then characterized for optimal temperature and pH and thermal and pH stability. Results indicated that the optimal temperature and pH of LiP were 30C and 4.0, respectively. This enzyme was stable between 20 and 30C for 2 hours (relative activities remain 94.17% and 89.10%, respectively) and pH from 2.0 to 4.0 for 3 hours (relative activities remain 78.97% and 97.87%), respectively. The LiP from strain TL4 showed potential application in environmental treatment and pre-treatment of cropby-products.

    References

    Bholay A.D., Borkhataria B.V., Jadhav P.U., Palekar K.S., Dhalkari M.V, P.M. Nalawade P.M. (2012). Bacterial Lignin Peroxidase: A Tool for Biobleaching and Biodegradation of Industrial Effluents. Universal Journal of Environmental Research and Technology, 2(1): 58-64.

    Chai C.C. (2008). Enhanced production of manganese peroxidase by Phanerochaete chrysosporium. Master thesis, University Sains Malaysia.

    Đặng Thị Cẩm Hà (2009). Nghiên cứu và xác định enzyme lignin peroxidase (LiP), manganese peroxidase (MnP) và laccase từ vi sinh vật phân hủy các hợp chất hữu cơ bền vững (POPs) và các hợp chất vòng thơm ô nhiễm khác. Đề tài cấp Viện KH&CNVN.

    Fakoussa, R.M. and Hofrichter M. (1999). Biotechnology and microbiology of coal degradation. Applied Microbiology and Biotechnology, 52: 25-40.

    Gomes E., Aguiar A.P., Carvalho C.C., Bonfa M.R., da Silva R., Boscolo M. (2009). Ligninases production by Basidiomycetes strains on lignocellulosic agricultural residues and their application in the decolorization of synthetic dyes. Brazilian Journal of Microbiology, 40: 31-39.

    Glumoff T., Lumoff., Harvey P.J., Leisoa S.A. (1990). Lignin peroxidase from Phanerochaete chrysosporium, Molecular and kinetic characterization of isozymes. Eur. J. Biochem, 187: 515-520.

    Hariharan S. and Nambisan P. (2013). Optimization of LigninPeroxidase, Manganese Peroxidase, and Lac Production from Ganoderma lucidum Under Solid State Fermentation of Pineapple Leaf. BioResources, 8(1): 250-270.

    https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/p6782enz.pdf

    Johansson T., Nyman P.O. (1993). Isozymes of lignin peroxidase and manganese (II) peroxidase from the white-rot basidiomycete Trametes versicolor: Isolation of enzyme forms and characterization of physical and catalyticproperties. Arch. Biochem. Biophys, 300: 49-56.

    Lesage L., Delattre M., Haon M., Thibault J.F. (1996). A two-step bioconversion process for vanillin production from ferulic acid. Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review combiningAspergillus niger andPycnoporus cinnabarinus.Journal of Biotechnology, 50(2): 107-113.

    Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1): 265-275.

    Mikiashvili N., Wasser S.P., Nevo E., Elisashvili V. (2011). Effects of carbon and nitrogen sources on Pleurotusostreatus ligninolytic enzyme activity.Curr Opin Biotech, 20: 348-357.

    Phí Quyết Tiến, Vũ Văn Lợi, Phan Thị Hồng Thảo, Phạm Thi Bích Hợp (2011). Tách dòng và biểu hiện lignin peroxidase H8 của chủngPhanerochae techrysosporium trongPichia pastoris. Tạp chí Công nghệ Sinh học, 9(2): 223-231.

    Sahadevan L.D.M., Misra S.C., Thankamani V. (2016). Characterization of lignin-degrading enzymes (LDEs) from a dimorphic novel fungus and identification of products of enzymatic breakdown of lignin. Springerlink.com, 3 Biotech 6: 5

    Tien M., Kirk T.K. (1984). Lignin-degrading enzyme from Phanerochae techrysosporium: Purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase. Proc Natl AcadSci USA, 81: 2280-2284.

    Trịnh Thị Thu Thủy, Nguyễn Văn Giang, Nguyễn Ngọc Bằng, Phạm Thu Trang (2015). Phân lập tuyển chọn các chủng nấm mốc sinh tổng hợp enzyme laccase từ gỗ mục. Tạp chí Khoa học và Phát triển, 7: 173-178.

    Võ Thị Ngọc Cẩm, Đỗ Thị Xuânvà cs. (2015). Phân lập và tuyển chọn một số dòng nấm bản địa phân hủy một số vật liệu hữu cơ từ nền đất thâm canh lúa tại xã Phong Hòa, huyện Lai Vung, tỉnh Đồng Tháp.Tạp chí Khoa học, Đại học Cần Thơ, 36: 1-11.