Evaluation of Quorum Sensing Communication between Pathogenic Bactria Causing Acute Hepatopancreatic Necrosis Disease and Vibrio Alginolyticus

Received: 01-08-2018

Accepted: 26-08-2018

DOI:

Views

0

Downloads

0

Issue: Vol. 16 No. 6 (2018)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Ha, T., Hanh, N., Viet, P., Van, P., & Hanh, T. (2024). Evaluation of Quorum Sensing Communication between Pathogenic Bactria Causing Acute Hepatopancreatic Necrosis Disease and Vibrio Alginolyticus. Vietnam Journal of Agricultural Sciences, 16(6), 571–577. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/471

Evaluation of Quorum Sensing Communication between Pathogenic Bactria Causing Acute Hepatopancreatic Necrosis Disease and Vibrio Alginolyticus

Tran Thi Thuy Ha (*) 1, 2 , Nguyen Thi Hanh 1 , Pham The Viet 1 , Phan Thi Van 1 , Truong Thi My Hanh 1

  • 1 Viện Nghiên cứu Nuôi trồng Thủy sản I
  • 2 Viện Nghiên cứu Nuôi trồng Thủy sản - Đình Bảng, Từ Sơn, Bắc Ninh

    • Keywords

    V. parahaemolyticus, V. alginolyticus, Quorum sensing, cinnamomum essential oil, AHPND

    Abstract


    Bacterial cell-to-cell communication(quorum sensing - QS) is associated with the virulence of pathogenic bacteria. The study was conducted to evaluatethe QS communication between Vibrioparahaemolyticus - pathogenic agent causing acute hepatopancreatic necrosis disease (AHPND) and V. alginolyticus- non-AHPND and, concurrently, to determine the role of cinnamon essential oil for QS between two bacterial species mentioned above. The methods used included traditional bacterial culture and PCR technique. Results showed that V. alginolyticus received virulence gene AHPND from V. parahaemolyticusunder the culture condition with three times of heat shock at 400C (5 min) and 700C (2 min) via QS mechanism. In addition, cinnamon essential oil with a dose of 0.1μL/600μL disrupted the QS communication system of bacteria, causing V. alginolyticusimpossible to receive virulence gene causing AHPND from V. parahaemolyticus.

    References

    Aparna Y., Narayanan L., Sarada J. (2014). Quorum quenching ability of dietary spice Cinnamomum verum on pathogenic bacteria. Int. J. Pharm. Sci. Res., 5: 5216-5223. doi:10.13040/IJPSR.0975-8232.5(12).5216-23

    Chong Y.M., Yan K., Yin W.F., Chan K.G. (2018). The Effects of Chinese Herbal Medicines on the Quorum Sensing-Regulated Virulence in Pseudomonas aeruginosa PAO1. Molecules, 23: 1-14.

    Coutteau P., Goossens, T. (2013). Funtional Feeds as effective strategies againts EMS. Aquac. Asia Pacific, 9: p. 31.

    Defoirdt, T. (2007). Quroum sensing disruption and the use of short-chain fatty acids and polyhydroxyalkanoates to control luminescent vibriosis (Doctoral dissertation, Ghent University).

    Defoirdt T., Sorgeloos, P. (2012). Monitoring of Vibrio harveyi quorum sensing activity in real time during infection of brine shrimp larvae. ISME J., 6: 2314-2319. doi:10.1038/ismej.2012.58

    Han J.E. (2017). Four AHPND strains identified on Latin American shrimp farms. http://advocate.gaalliance.org/four-ahpnd-strains-identified-on-latin-american-shrimp-farms/.

    Hilton, T., Rosche, T., Froelich, B., Smith, B., Oliver, J. (2006). Capsular polysaccharide phase variation in Vibrio vulnificus. Appl. Environ. Microbiol., 72: 6986-6993. doi:10.1128/AEM.00544-06

    Kamruzzaman M., Udden S.M.N., Cameron D.E., Calderwood S.B., Nair G.B., Mekalanos J.J., Faruque S.M. (2010). Quorum-regulated biofilms enhance the development of conditionally viable, environmental Vibrio cholerae. Proc. Natl. Acad. Sci., 107: 1588-1593. doi:10.1073/pnas.0913404107

    Kondo H., Van P.T., Dang L.T., Hirono I. (2015). Draft Genome Sequence of Non-Vibrio parahaemolyticus Acute Hepatopancreatic Necrosis Disease Strain KC13.17.5, Isolated from Diseased Shrimp in Vietnam. Genome Announc 3. doi:10.1128/genomeA.00978-15

    McDougald D., Lin W., Rice S., Kjelleberg S. (2006). The role of quorum sensing and the effect of environmental conditions on biofilm formation by strains of Vibrio vulnificus. Biofouling, 22: 133-144. doi:10.1080/08927010600743431

    Priya K., Yin W.F., Chan K.G. (2013). Anti-quorum sensing activity of the traditional chinese herb, Phyllanthus amarus. Sensors (Switzerland), 13: 14558-14569. doi:10.3390/s131114558

    Rasch M., Buch C., Austin B., Slierendrecht W.J., Ekmann K.., Larsen J.C.J., Riedel K. Eberl, L., Givskov, M., Gram, L. (2004). An inhibitor of bacterial quorum sensing reduces mortalities caused by Vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). Syst. Appl. Microbiol., 27: 350-359.

    Schauder S., Bassler B.L. (2001). The languages of bacteria. Genes Dev. doi:10.1101/gad.899601

    Waters, C.M., Bassler, B.L. (2005). Quorum Sensing : Communication in Bacteria. Annu. Rev. Cell Dev. Biol., 21: 319-346. doi:10.1146/annurev.cellbio. 21.012704.131001

    Yap P.S.X., Krishnan T., Chan K.G., Lim S.H.E. (2015). Antibacterial mode of action of Cinnamomum verumbark essential oil, alone and in combination with piperacillin, against a multi-drug-resistant Escherichia coli strain. J. Microbiol. Biotechnol., 25: 1299-1306. doi:10.4014/jmb.1407. 07054.