Isolation and Identification of Biological Characteristicsof Porcine Epidemic Diarrhea Virus

Received: 08-12-2017

Accepted: 21-05-2018

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Issue: Vol. 16 No. 3 (2018)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Hoa, N., Lan, N., Lam, T., Thau, T., & Hanh, N. (2024). Isolation and Identification of Biological Characteristicsof Porcine Epidemic Diarrhea Virus. Vietnam Journal of Agricultural Sciences, 16(3), 257–267. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/441

Isolation and Identification of Biological Characteristicsof Porcine Epidemic Diarrhea Virus

Nguyen Thi Hoa (*) 1 , Nguyen Thi Lan 1 , Truong Quang Lam 1 , Trinh Dinh Thau 1 , Ngo Thi Hanh 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Porcine epidemic diarrhea virus, virus isolation, biological characteristics

    Abstract


    Porcine Epidemic Diarrhea Virus(PEDV) causesacute diarrhea in pigs and this is especially dangerous for piglets. Isolation of PEDV is a pre-requisite for research on production of vaccinesor bioformulationsin the prevention and treatment of the disease.Small intestinesof pigs diagnosed positively with PEDV based on RT-PCR method and vero cell lines were used for this study. PEDV isolationswere determined for the viral titre and the growth curve. A total of 13 PEDV strains were successfully isolated from83diseasesamples. The medium containing 10µg/ml trypsinwas suitable for viral isolation. PEDV was permissive for replication and caused disease at the beginning ofthe second culture passage. Infected vero cell cultures exhibited characteristics CPE by cell-fusion and syncytial formation. CPE occured at 12 hours post infection and the cellswerecompletely destroyed after 36 to 48 hours of infection. In thefirst passage,CPE viral titre ranged from 103,4to 105,7TCID50/mlbut after4 culture passages,theviral titre increasedfrom 105,3 to 107,3TCID50/ml.

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