Received: 24-02-2017
Accepted: 15-05-2018
DOI:
Views
Downloads
How to Cite:
Pathological Characteristics of Kept WildPigsInfected with Porcine Epidemic Diarrhea Virus (PEDV)
,
Nguyen Vu Son
2
,
Pham Hong Ngan
2
,
Nguyen Huu Nam
3
Keywords
Diarrhea, PED (Porcine epidemic diarrhea)virus, pathological characteristics, wild boar
Abstract
Thirty-five piglets aged from 1 to 4 weeks were collected from wild pigfarms in Ha Giang province. The RT-PCR was used to confirm porcine epidemic diarrhea (PED) virus. The results showedthat the proportion of positive samples was74.3% and 54.3% for small intestine and mesenteric lymph node andfor fecal samples, respectively. The main clinical signs of PED included weakness, depression, and diarrhea with loose, fishy, and yellow stools (100%)andweight loss (80.8%). Suckling pigs showed anorexia, huddling together, laying on the sow (76.9%), dehydration (69.2%), loss of appetite (57.7%), hypothermia and heaving (53.8%).The autopsies showed distended stomach containing curd and undigested food (100%),thinness of the wall of small intestine and watery yellow translucent content in small intestine (100.0%),hyperemia and hemorrhage of mesenteric lymph nodes (80.8%),enlargement of lymph nodes in other organs (69.2%); pale liver (69.2%),pneumonia (61.5%),splenic congestion, and gallbladder enlargement (57.7%). Microscopic lesions included small intestinal congestion, hemorrhage, severe villous atrophy, scraped mucosa, lymphoid follicles hyperplasia, inflammatory cell infiltration, degeneration, and necrosis. Congestion and inflammatory cells infiltration were observed in liver, spleen, and lung. In addition, hepatocellular degeneration and lipid deposition were observed in the liver.
References
Bộ Khoa học & Công nghệ (2010). Tiêu chuẩn TCVN 8402:2010 - Bệnh động vật, Quy trình mổ khám, ban hành theo quyết định 2339/QĐ-BKHCN.
Nguyễn Văn Điệp, Nguyễn Thị Lan, Nguyễn Thị Hoa, Yamaguchi (2014). Một số đặc điểm dịch tễ và bệnh lý của bệnh tiêu chảy thành dịch trên lợn ở một số tỉnh phía Bắc Việt Nam. Tạp chí Khoa học kỹ thuật Thú y, 21(2): 43-55.
Nguyễn Tất Toàn, Nguyễn Đình Quát, Trịnh Thị Thanh Huyền, Đỗ Tiến Duy, Trần Thị Dân, Nguyễn Thị Phước Ninh và Nguyễn Thị Thu Năm (2012). Phát hiện virus gây bệnh tiêu chảy cấp (PEDV) trên heo ở các tỉnh miền Đông Nam Bộ. Tạp chí Khoa học kỹ thuật Thú y, 19(5): 26-30.
Bertschinger, Fairbrother, Nielson, Pohlenz (1992). Escherichia coli infection. Diseases of Swine. 7th edition. Iowa State Unirversity Press, Ames, Iowa 50010. p. 504.
Chang, S.H., Bae, J.L., Kang, T.J., Kim, J., Chung, G.H., Lim, C.W., Laude, H., Yang, M.S. and Jang, Y.S. (2002). Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus. Molecules and Cells, 14: 295-299.
Do Tien Duy, Nguyen Tat Toan, Suphasawatt Puranaveja, Roongroje Thanawongnuwech (2011). Genetic Characterization of Porcine Epidemic Diarrhea Virus (PEDV) isolates from southern Vietnam during 2009 - 2010 outbreaks. Thai J Vet Med., 41(2): 55-64.
Gregory W. Steven, hai Hoang, Kent J. Schwartz, Eric R. Burrough, Dong Sun, Darin Madson, Vickie L. Cooper, Angela, Pillatzki, Phlip Gauger, Beverly J. Schmitt, Leo G. Koster, Mary L. Killan and Kyoungjin J. Yoon (2013). Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesion, and viral. Journal of Veterinary Diagnostic Investigation, 25(5): 649-654.
Hak- Mo LEE , Beom - Jun, Joo - Ho TAE, Chang - Hee KWEON, Yong-Soon LEE and Jae-Hak PARK (2000). Detection of Porcine epidemic diarrhea virus by Immunohistochemistry with Recombinant Antibody Produced Phages J. Vet. Med. Sci., 62(3): 333-337.
Park B, Daesub Song (2012) Porcine epidemic diarrhea. virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines. Virus Genes, 44: 167-175.
Park, S.J., Moon, H.J., Yang, J.S., Lee, C.S., Song, D.S., Kang, B.K. and Park, B.K. (2007). Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea. Virus genes, 35: 321-332.
Pensaert M.B. and Yeo, S.G. (2006). Porcine epidemic diarrhoea. In: B.E. Straw., J.J. Zimmerman., S. D’Allaire and D.J. Taylor (Eds.). Diseases of swine, 9th ed. Oxford: Wiley- Blackwell. pp. 367-372.
Pensaert M.B (1992). Chapter 22 Porcine Epidemic Diarrhea. Diseases of Swine 7th edition Iowa State Unirversity Press, Ames, Iowa 50010, pp. 293-298.
Pensaert M.B, Debouck P, Reynolds DJ (1981). An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV 777, and several coronaviruses. Arch Virol., 68(1): 45-52.
Pensaert, M.B. 1981. Porcine epidemic diarrhea, p.344-346, in A.D. Leman et al. (ed.) Diseases of swine, 5th ed. The Iowa State University Press, Ames.
Pospichil A, Hess RG, Bachmann PA 1981. Light microscopy and ultrahistology of intestinal changes in pigs ifected with epizootic virus diarrhea (EVD): comparíon with transmissible gastroenteritis (TGE) virus and porcine rotavirus infections. Zentralbl Veterinaermed [B] , 1981; 28:564 - 577
Prophet, E.B. and A.F.I.O. Pathology.(1992). Laboratory methods in histotechnology, American Registry of Pathology.
Puranaveja, S., Poolperm, P., Lertwatcharasarakul, P., Kesdaensakonwut, S., Boonsoongner, A., Urairong, K., Kitikoon, P., Choojai, P., Kedkovid, R., Teankurn, K., and Thanawongnuwech, R. (2009). Chinese-like strain of Porcine epidemic diarrhea virus, Thailand. Emerg Infect Dis., 15(7): 1112-1115.
Sueyoshi M, Tsuda T, Yamazaki K, Yoshida K, Nakazawa M, Sato K, Minami T, Iwashita K, Wantanabe M, Suzuki Y (1995). An immunohisochemical investigation of porcine epidemic diarrhoea. Jcomp Pathol., 113(1): 59-67.
Wilcok, Schwartz (1992). Chapter 46, Salmonellosis, Diseases of Swine, 7th edition, Iowa State Unirversity Press, Ames, Iowa 50010. p. 573
Wood EN, (1977) An apparently new syndrome of Porcine Epidemic Diarrhoea. Vet. Rec, 100: 243-244.