Isolation of Bacteria Caused Hemorrhagic Disease for Catfish (Ictalurus punctatus) in The Northern Parts of Vietnam

Received: 28-02-2017

Accepted: 24-04-2017

DOI:

Views

4

Downloads

0

Issue: Vol. 15 No. 4 (2017)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Trang, T., Dung, N., & Hoai, T. (2024). Isolation of Bacteria Caused Hemorrhagic Disease for Catfish (Ictalurus punctatus) in The Northern Parts of Vietnam. Vietnam Journal of Agricultural Sciences, 15(4), 346–354. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/366

Isolation of Bacteria Caused Hemorrhagic Disease for Catfish (Ictalurus punctatus) in The Northern Parts of Vietnam

Trinh Thi Trang (*) 1 , Nguyen Thi Dung , Truong Dinh Hoai

  • 1 Khoa Thủy sản, Học viện Nông nghiệp Việt Nam

    • Keywords

    Isolation, haemorrhagic, catfish, antibiotic

    Abstract


    Cat fish, Ictalurus punctatusis increasingly cultured in the Northern part of Vietnam.However, the diseaseson the fish have not yet studied extensively with limited literature. The study was implemented to isolate pathogenic bacteria causing hemorrhagic disease in catfish and to find out highly effective antibiotics for disease treatment. The result showed that the external signs of the diseases are black skin with lossing mucus and haemorrhagic spots in the head part, fins, and abdomen. The internal signs showed damaged kidney and liquid accumulated abdomen. Thirteen rod shape, gram negative bacterial strains that grew in selected R-S medium were isolated from diseased fish. These strains were identified as Aeromonas hydrophila. Histopathological analysis of hemorrhagic diseased catfish revealed structural changes with slight necroses in muscle and gill filaments. There were many necrotic areas and granulomatous lesions on the liver, kidney and spleen, hydrophytic kidney and spleen tissues. After 2 day period of infection,bacterial strainsCL18, CL20, CL24 and CL26 at 108CFU/fish caused hemorrhagic septicemia in healthy catfish similarly to those in natural infection. Among 7 types of antibiotics, Doxycylin most sensitive with 13 tested isolates. Therefore, the use of Doxycylin on catfish haemorrhagic disease treatment is highly effective.

    References

    Bauer, R.W., Kirby, M.D.K., Sherris, J.C. and Turck, M. (1966). Antibiotic susceptibility testing by standard single disc diffusion method. Am. J. Clin. Pathol.,45: 493-496.

    Buller, N.B. (2004). Bacteria from fish and other aquatic animal - Apractical dentification mannual. CABI publishing.

    Cabello, F.C. (2006) Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ. Microbiol.,8: 1137-1144.

    Đặng Thị Hoàng Oanh và Nguyễn Đức Hiền (2012). Phân lập và xác định khả năng gây bệnh xuất huyết trên lươn đồng (Monopterus albus) của vikhuẩn Aeromonas hydrophila. Tạp chí Khoa học, Trường đại học Cần thơ, 22: 173-182.

    Đặng Thụy Mai Thy, Trần Thị Thủy Cúc, Nguyễn Châu Phương Lam, Nguyễn Đức Hiền và Đặng Thị Hoàng Oanh (2012). Đặc điểm mô bệnh học cá rô (Anaba stestudineus) vikhuẩn Aeromonas Hydrophyla và Streptococcus sp. trong điều kiện thực nghiệm. Tạp chí Khoa học, 22: 183-193.

    Depaola, A., Pauline A. Flynn, R. Merrill McPhearson and Stuart B. Levy (1988). Phenotypic and Genotypic Characterization of Tetracycline and Oxytetraxycline Resistance Aeromonas hydrophila from Cultured Channel Catfish (Ictaluruspunctatus) and their Environments (1988). Applied and Environmental Microbiology, pp. 1861-1863.

    Dung, T.T., Haesebrouck, F., Tuan, N.A., Sorgeloos, P., Baelem M. and Decostere, A. (2008). Antimicrobial susceptibility pattern of Edwarsiella ictaluri isolate from natural outbreaks of bacillary necrosis of Pangasianodon hypophthalmus in Vietnam. Microbial Drug Resistance, 14(4): 311-316.

    Kim Văn Vạn (2001). Bệnh của cá trắm cỏ nuôi lồng. Tạp chí Khuyến ngưViệt nam, 5: 30-31.

    Mohamed N., Kindon Sung, Saeed A. Khan, Ashraf A. Khan, and Roger Steele (2006). Biochemical and Molecular Characterization of Tetracycline-Resistance Aeromonas veronii Isolates from Catfish. Applied and Environmental Microbiology, pp. 6461-6466.

    Nguyễn Thị Ngọc Huyền và Đặng Thị Hoàng Oanh (2015). Đặc điểm mô bệnh học của cá lóc (Channa striata) bệnh xuất huyết và bệnh gan thận mủ. Tạp chí Khoa học Trường đại học Cần Thơ, 45b: 93-100.

    Popoff, M. (1984). Genus III Aeromonas. In Bergey’s Manual of Determinative Bacteriology, N.R. and Holt, J.G., 1: 545-548.

    Samira Sarter, Hoang Nam Kha Nguyen, Le Thanh Hung, Jérôme Lazard, and Didier montet (2007). Antibiotic Resistace in Gram-negative bacteria isolated from farmed catfish. Food Control, 18: 1391-1396.

    West, P.A., P.R. Brayton, T.N. Bryant and R.R. Colwell (1986). Numerical taxonomy of Vibrios isolated from aquatic environments. International Journal of Systematic Bacteriology, 36(4): 531-543.