Preliminary Identification of Porcine Deltacoronavirus (PDCoV) from Pigs in Hanoi and Thai Binh

Received: 13-06-2017

Accepted: 24-04-2017

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Issue: Vol. 15 No. 2 (2017)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Phan, L., Phuong, C., Hieu, D., Quyen, L., & Thach, P. (2024). Preliminary Identification of Porcine Deltacoronavirus (PDCoV) from Pigs in Hanoi and Thai Binh. Vietnam Journal of Agricultural Sciences, 15(2), 171–177. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/349

Preliminary Identification of Porcine Deltacoronavirus (PDCoV) from Pigs in Hanoi and Thai Binh

Le Van Phan (*) 1 , Cao Thi Bich Phuong 1 , Dong Van Hieu 1 , Le Dinh Quyen 2 , Pham Ngoc Thach 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Công ty cổ phần Phát triển công nghệ nông thôn (RTD)

    • Keywords

    Porcine deltacoronavirus, diarrhea, pig, RT-PCR

    Abstract


    Reverse transcription - polymerase chain reaction (RT-PCR) and sequencing methods were used to identify porcine deltacoronavirus (PDCoV) in eighteen fecal and intestine samples of diarrhae piglets in four farms in Thai Binh and Hanoi in 2015. The diagnostic results showed that 15/18 (83.33%) samples were possitive with PDCoV. The nucleotide and deduced amino acid analysis based on the nucleocapsid (N) gene sequence indicated that nucleotide and aa sequences of PDCoV/VN/TB1/2015 were homologous to Dong Nai isolate (VN-1215, isolated in 2015) at 99% and 99.5%, respectively. The phylogenetic analysis indicated that PDCoV/VN/TB1/2015 and VN-1215 strain were closely related to PDCoV isolates found in 2012 in Hongkong and in 2014 in China.

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