Lead-Induced Oxidative Stress and Defense Responseof Soybean ‘DT84’

Received: 14-01-2015

Accepted: 22-07-2015

DOI:

Views

0

Downloads

0

Issue: Vol. 13 No. 5 (2015)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Chung, M., & Toan, T. (2024). Lead-Induced Oxidative Stress and Defense Responseof Soybean ‘DT84’. Vietnam Journal of Agricultural Sciences, 13(5), 214. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/214

Lead-Induced Oxidative Stress and Defense Responseof Soybean ‘DT84’

Mai Van Chung (*) 1 , Tran Ngoc Toan 1

  • 1 Khoa Sinh học, Trường đại học Vinh

    • Keywords

    CAT, hydrogen peroxide, lead, oxidative stress, soybean, superoxide, SOD

    Abstract


    Oxidative stress in root of soybean [(Glycine max (L.) Merr.] cv. DT84’ was induced by exposure to Pb2+ ion. The reactive oxygen species (ROS) products such as hydrogen peroxide (H2O2), superoxide anion radical (O2.-) were rapidly produced 6-12 hours after treatment with Pb2+. The enhancement of the ROS was closely dependent upon concentrations of Pb2+. An increase in content of thiobarbituric acid reactive substances (TBARS) - product of membrane lipid peroxidation, and the high percentage of injury (13.89-25.03%) indicated that the cellular damage was resulted from oxidative stress. The induction of superoxide dismutase (SOD) and catalase (CAT) in roots of soybean cv. DT84 as the defense response of soybean via these antioxidative enzymes reduced the concentration H2O2 and O2.- 24 hours following Pb2+ treatment, therefore, contributing to control oxidative stress in soybean roots.

    References

    Ahmad P., Sarwat M. and Sharma S. (2008). Reactive oxygen species, antioxidants and signaling in plants. J. Plant Biol., 51: 167-173.

    Beauchamp C. and Fridovich I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem.,44: 276-287.

    Becana M., Aparicio-Tejo P., Irigoyen J.J. and Sanchez-Diaz M. (1986). Some enzymes of hydrogen peroxide metabolism in leaves and root nodules of Medicago sativa. Plant Physiol., 82: 1169-1171.

    Bhattacharjee S. (2005). Reactive oxygen species and oxidative burst: Roles in stress, senescence and signal transduction in plants. Curr. Sci., 89: 1113-1121.

    Bradford M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem.,72: 248-254.

    Mai Văn Chung, Nguyễn Đức Diện, Nguyễn Đình San (2014). Phản ứng siêu nhạy cảm ở rễ cây đậu tương Nam Đàn đối với chì. Tạp chí Khoa học và Phát triển, 12(7): 1023-1028.

    Dey S.K., Dey J., Patra S. and PothalD. (2007). Changes in the antioxidative enzyme activities and lipid peroxidation in wheat seedlings exposed to cadmium and lead stress. Braz. J. Plant Physiol., 19(1): 53-60.

    Dhindsa R.S., Plumb-Dhindsa P. and Thorpe T.A. (1981). Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decrease levels of superoxide dismutase and catalase. J. Exp. Bot., 32:93-101.

    Doke N. (1983). Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components. Physiol.Plant Pathol.,23: 345-357.

    Hassan M. and Mansoor S. (2014). Oxidative stress and antioxidant defense mechanism in mung bean seedlings after lead and cadmium treatments. Turk. J. Agric. For., 38: 55-61.

    Heath R.L. and Packer L. (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys.,125: 189-198.

    Maffei M.E., Mithӧfer A. and Boland W. (2007). Insects feeding on plants: Rapid signals and responses preceding the induction of phytochemical release. Phytochemistry, 68: 2946-2959.

    Mai V.C., Bednarski W., Borowiak-Sobkowiak B., Wilkaniec B., Samardakiewicz S. and Morkunas I. (2013). Oxidative stress in pea seedlings leaves in response to Acyrthosiphon pisum infestation. Phytochemistry, 93: 49-62.

    Malecka A., Jarmuszkiewicz W. and Tomaszewska B. (2001). Antioxidative defense to lead stress in subcellular compartments of pea root cells. Acta Biochim. Pol., 48(3): 687-98.

    Malecka A., Piechalak A. and Tomaszewska B. (2009). Reactive oxygen species production and antioxidative defense system in pea root tissues treated with lead ions: the whole roots level. Acta Physiol. Plant., 31: 1053–1063.

    Mittler R., Vanderauwera S., Gollery M. and Van Breusegem F. (2004). Reactive oxygen gene network of plants. Trends Plant Sci., 9: 490-498.

    Radville L., Chaves A. and Preisser E.L. (2011). Variation in plant defense against invasive herbivores: evidence for a hypersensitive response in Eastern Hemlocks (Tsuga canadensis). J. Chem. Ecol., 37: 592-597.

    Rodriguez-Serrano M., Romero-Puertas M.C., Pazmino D.M., Testillano P.S., Risueno M.C., del Rio L.A. and Sandalio L.M. (2009). Cellular response of pea plants to cadmium toxicity: Cross talk between reactive oxygen species, nitric oxide, and calcium. Plant Physiol., 150: 229- 243.

    Rueda A., Roman Y., Lobo M. and Pelaez C. (2011). Analysis of tomato seedling cell death in response to copper and paraquat induction. Trop. Plant Pathol.,36: 169-177.

    Skorzynska-Pilot E. (2006). Lipid peroxidation in plant cells: its physiological role and change sunder effecst of heavy metal stress. Acta Soc. Bot. Pol., 76: 49-54

    Sullivan Y.C. (1971). Techniques of measuring plant drought stress. In: Larson K.L. and Eastin J.D., (Eds.), Drought injury and resistance in crops. Crop Science Society of America, Madison, WI, p. 1-8.

    Wojtaszek P. (1997). Mechanisms for the generation of reactive oxygen species in plant defence response. Acta Physiol. Plant., 19: 581-589.

    Zhu-Salzman K., Salmon R.A., Ahn J.-E., and Koiwa H. (2004). Transcriptional regulation of sorghum defense determinants against a phloem-feeding aphid. Plant Physiol., 134: 420-431.