Genotyping of Clostridium perfringens Isolated from Cattle and Pigs with Diarrhea in Hanoiand Surrounding Areas, Vietnam

Received: 14-03-2012

Accepted: 28-07-2012

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Issue: Vol. 10 No. 4 (2012)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Le, H., Thuy, D., & Hien, N. (2024). Genotyping of Clostridium perfringens Isolated from Cattle and Pigs with Diarrhea in Hanoiand Surrounding Areas, Vietnam. Vietnam Journal of Agricultural Sciences, 10(4), 627–632. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1678

Genotyping of Clostridium perfringens Isolated from Cattle and Pigs with Diarrhea in Hanoiand Surrounding Areas, Vietnam

Huynh Thi My Le (*) 1 , Do Ngoc Thuy 2 , Nguyen Ba Hien 1

  • 1 Bộ môn Vi sinh vật - Truyền nhiễm, Khoa Thú y, Trường Đại học Nông nghiệp Hà Nội
  • 2 Bộ môn Vi trùng, Viện Thú y quốc gia

    • Keywords

    Clostridium perfringens, cattle, pigs, diarrhea, multiplex PCR

    Abstract


    Diarrhea is a major cause of economic loss in cattle and pig farming. The aim of this study was epidemiological survey of prevalence and molecular typing of C. perfringens isolates associated with diarrhea in cattle and pigs. The study was carried out from 2007 to 2010 in Hanoi and surrounding areas (including Bac Ninh and Vinh Phuc provinces). The biochemical properties of isolated C. perfringens were tested. The results showed that: all isolates of C. perfringens had biochemical properties as described before. PCR typing of isolates was carried out by multiplex PCR. C. perfringens isolated from fecal samples of diarrheic cattle were type A (57.34%), type D (41.33%) and type C (1.33%); whereas all C. perfringens isolated from fecal samples of healthy cattle, diarrheic and healthy pigs were type A. The prevalence of cpe and cpb2 varied with isolated genotypes. There was a significant association between cpb2 positive C. perfringens isolates and diarrhea in pigs. Of the 304 isolates from pigs with diarrhea examined, 138 (45.39%) were positive for the cpb2 gene and 52 (17.11%) were positive for the cpe and cpb2 genes, whereas none of the isolates from healthy pigs were positive for the cpb2 gene.

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