In vitroMicropropagation of Wild Orchid Dendrobium nobileLindl.

Received: 26-08-2013

Accepted: 15-11-2013

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Issue: Vol. 11 No. 7 (2013)

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NÔNG HỌC

How to Cite:

Lan, V., & Anh, N. (2024). In vitroMicropropagation of Wild Orchid Dendrobium nobileLindl. Vietnam Journal of Agricultural Sciences, 11(7), 917–925. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1667

In vitroMicropropagation of Wild Orchid Dendrobium nobileLindl.

Vu Ngoc Lan (*) 1 , Nguyen Thi Ly Anh 1

  • 1 Viện Sinh học Nông nghiệp, Trường Đại học Nông nghiệp Hà Nội

    • Keywords

    Dendrobium nobileLindl., seed capsules, propagation, protocorm

    Abstract


    Studies of micropropagation of D. nobileLindl. were conducted in order to conserve and increase the genetic pool of this precious orchid species. The results showed that the suitable materials used for micropropagation were 5 month old seed capsules. The most suitable medium for germination and protocorm formation was MS+100ml coconut juice+10g saccharose+6.0g agar/liter. For in vitro propagation by traditional method, the optimal medium for propagation of protocorms was KC+100ml coconut juice+10g saccharose+6.0g agar/liter. The most appropriate medium for rapid in vitropropagation of shoots was MS+100ml coconut juice+10g saccharose+6.0g agar/liter. For in vitro propagation by improved method using liquid medium and cotton buttons combined with shaking or using liquid medium and ventilative buttons (cellulose acetate) combined with shaking had increased multiplication rate of protocorms (1.9 and 2.3 fold in comparing with the control). The propagation using solid medium with ventilation buttons reduced 25% of saccharose added to medium, and increased the multipliecation rate of protocorms up to 1.4 fold in comparison with propagation by the classical method. Similarly, rapid in vitro propagation using bioreactor redued the time of propagation by half. The most optimal medium used regenerating intact plants of D. nobilewas RE+10g saccharose +0.5g activated carbon+6.0g agar/liter combined with light intensity of 2300lux.

    References

    Nguyễn Tiến Bân và nhiều tác giả (2007). Sách Đỏ Việt Nam, Phần Thực vật; NXB. Khoa học Tự nhiên và Công nghệ, Hà Nội.

    Lê Văn Hoàng (2008). Giáo trình nuôi cấy mô tế bào thực vật. Đại học Đà Nẵng

    Trần Văn Huân, Văn Tích Lượm (2007). Kỹ thuật nuôi trồng cây lan. NXB thành phố Hồ Chí Minh.

    Dương Đức Huyến (2007). Thực vật chí Việt Nam, 9- Họ lan (Orchidceae). NXBKH Kỹ thuật

    Nguyễn Văn Song 2011). Nhân nhanh in vitro lan Kim Điệp (Dendrobium chrysotoxum)-một loài lan rừng có nguy cơ tuyệt chủng. Tạp chí khoa học ĐH Huế 64:127-136.

    Đào Thị Thanh Vân, Đặng Thị Tố Nga (2008). Giáo trình hoa lan. NXB Nông nghiệp, Hà Nội.

    Kauth P. (2005). In vitro seed germination and seedling development of Calopogon tuberosus and Sacoila lanceolata var. lanceolata: Two Florida native terrestrial orchids. Master thesis, University of Florida

    Kusumoto and Furukawa (1977). Effect of Organic Matter on the Growth of Cymbidium Protocorms Cultured in vitro, Japan. Soc. Hort. Sci. 45 (4): 421-426.

    Shu Fung Lo, Satish Manohar Nalawade, Chao Lin Kuo, Chung Li Cheng and Hsin Sheng Tsay (2004). Asymbiotic germination of immature seeds, plantlet development and ex vitro establishment of plants of Dendrobium tosaense makino-a medicinally important orchild, In vitro Cellular and Developmental Biology - Plant 40 (5): 528-535.

    Tawaro Supavadee, Suraninpong Potjamarn and Chanprame Sontichai (2008). Germination and Regeneration of Cymbidium findlaysonianum Lindl.on a Medium Supplemented with Some Organic Sources. Walailak J Sci & Tech 5 (2): 125-135.