In vitro Propagation of Morinda officinalisHow

Received: 15-04-2013

Accepted: 18-06-2013

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Issue: Vol. 11 No. 3 (2013)

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NÔNG HỌC

How to Cite:

The, H., Thao, N., Thao, N., & Thuy, N. (2024). In vitro Propagation of Morinda officinalisHow. Vietnam Journal of Agricultural Sciences, 11(3), 285–292. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1629

In vitro Propagation of Morinda officinalisHow

Hoang Thi The (*) 1 , Nguyen Thi Phuong Thao 2 , Ninh Thi Thao 2 , Nguyen Thi Thuy 2

  • 1 Trung tâm Khoa học và sản xuất lâm nông nghiệp Quảng Ninh
  • 2 Khoa Công nghệ sinh học – Trường Đại học Nông nghiệp Hà Nội

    • Keywords

    BA, in vitro, Kinetin, Morinda officianalisHow, propagation

    Abstract


    Thestudy was conducted to establish the procedure for in vitropropagation of Morinda officianalisHow using stem segments. The stem segments were initiate cultured on MS medium containing 0,25 mg/l Kinetinand 1,0 mg/l BA, on which 96,6 % of explants induced shoots after 30 days of culture. The highest shoot multiplication rate (10,13 times) was obtained on MS medium supplemented with 3,0 mg/l BA; 0,2 mg/l IBA and 10,0 mg/l Riboflavin after 45 days of culture. Root induction medium was ½ MS containing 0,2 mg/l IBA and 0,4 g/l activated charcoal.The average rooting frequency was 100 % with a mean of 3.5 roots/shoot within 30 days. In vitroplantlets after 35-days period of rooting were the most appropriateforsuccessfulacclimatization in greenhouse. The highest frequency of acclimatized plantletswas 96,2 %inthe organic substrate containing 50 % of coconut fiber and 50 % of mushroom-production waste materials, the plantlets showed good growth and development.

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