Investigation of Microbial Composition and Probiotic Properties of Commercial Probiotic Products

Received: 13-12-2013

Accepted: 19-02-2014

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Nghiem, D., Huyen, N., Huong, N., Linh, T., Ha, N., Hao, T., & Linh, N. (2024). Investigation of Microbial Composition and Probiotic Properties of Commercial Probiotic Products. Vietnam Journal of Agricultural Sciences, 12(1), 65–72. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1568

Investigation of Microbial Composition and Probiotic Properties of Commercial Probiotic Products

Dang Xuan Nghiem (*) 1 , Nguyen Thi Huyen 1 , Nguyen Thi Thu Huong 1 , Trinh Thi Thuy Linh 1 , Nhu Thi Ha 1 , Trinh Thi Hao 1 , Nguyen Thanh Linh 1

  • 1 Khoa Công nghệ Sinh học, Đại học Nông nghiệp Hà Nội
  • Keywords

    Bacillus, Bifidobacterium, Lactobacillus, probiotics, Streptococcus

    Abstract


    In this study, microbial compositions and probiotic properties of 24 commercial probiotic products for human available in 2012 in Hanoi were evaluated. According to the labeled information, all probiotic bacteria in those products belonged to Bacillus, Lactobacillus, Streptococcus and Bifidobacteriumgenera, wherebyL. acidophiluswasmost frequently found with an approximatevalue of 80%. Plate counting method revealed that 13 out of 24 investigated products had lower live cell numbers than those claimedon the labels or in the statustory regulations. In addition, no Bifidobacteriumstrains could beisolated from any products labeled with their names. All 54 isolated strains showedgood adherence to chicken mucus, strong gastric acidic fluid tolerance and high resistance to bile salts. Their antibiotic resistance towards six antibiotics and their antagonistic effect against three common pathogenic bacterial species differed largely with 59.3% of the strains resistant to at least one antibiotic; 5 strains could inhibited at least one pathogenic bacterial species and 9 strains can inhibited all three pathogens.

    References

    Allen, S.J., Martinez, E. G., Gregorio, G.V., Dans, L.F. (2010). Probiotics for treating acute infectious diarrhoea. Cochrane Database Syst Rev. 11: CD003048

    Araya, M., Morelli, L., Reid, G., Sanders, M.E., and Stanton, C. (2002). Joint FAO/WHO Working Group Report on Guidelines for the evaluation of probiotics in food, London, Ontario.

    Arthur, C., Ouwehand and Salminen, S. (2003). In vitro Adhesion Assays for Probiotics and their in vivo Relevance: A Review. Microbial Ecology in Health and Disease 2003; 15: 175-184

    Arunachalam, K., Gill, H. S., Chandra, R. K. (2000). Enhancement of natural immune function by dietary consumption of Bifidobacterium lactis (HN019). Eur J Clin. 54: 263-267.

    Balayan, M.A., Susanna, S., Mirzabekyan, Isajanyan, M., Pepoyan, Z. S., Trchounian, А. H., Pepoyan, А. Z. and Bujdakova, H. (2010). Some Peculiarities of Growth and Functional Activity of Escherichia coli Strain from Probiotic Formula “ASAP”. World Academy of Science, Engineering and Technology. 44.

    Bengmark, S. (2000). Colonic food: pre- and probiotics. Am J Gastroenterol. 95: 5-7.

    Boonkumklao, P., Kongthong, P., & Assavanig, A. (2006). Acid and bile tolerance of Lactobacillus thermotolerans, a novel species isolated from chicken feces. Kasetsart J Nat Sci. 40: 13-17.

    Chateau, N., Castellanos, I. and Deschamps, A. M. (1993). Distribution of pathogen inhibition in the Lactobacillus isolates of a commercial probiotic consortium. J. Appl. Bacteriol. 74: 36-40.

    Corcoran, B. M., Stanton, C., Fitzgerald, G. F., and Ross, R. P. (2005). Survival of Probiotic Lactobacillus in Acidic Environments Is Enhanced in the Presence of Metabolizable Sugars. Appl Environ Microbiol. 71(6): 3060-3067.

    Dunne, C., O’Mahony, L., Murphy, L., Thornton, G., Morrissey, D., O’Halloran, S., Feeney, M., Flynn, S., Fitzgerald, Daly, C., Kiely, B., O’Sullivan, G.C., Shanahan, F., Collins, J.K. (2001). In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am. J. Clin. Nutr. 73 (2): 386-392.

    Fuller, R. (1997). Probiotics 2: applications and practical aspects. London: Chapman & Hall.

    Fukuda S, Toh H, Hase K, Oshima K, Nakanishi Y, et al. (2011) Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature. 469: 543-547

    Ganan, M., Martínez-Rodríguez, A.J.,Carrascosa, A.V., Vesterlund, S., Salminen, S. and Satokari, R. (2012). Interaction of Campylobacter spp. and human probiotics in chicken intestinal mucus. Zoonoses and Public Health. 60 (2): 141-148.

    Gueimonde, M., Sánchez, B., Clara, G., de los Reyes-Gavilánand Abelardo Margolles(2013). Antibiotic resistance in probiotic bacteria. Frontiers in Microbiology (4) 202. doi: 10.3389/fmicb.2013.00202.

    Gueimonde, M., Sánchez, B., G de Los Reyes-Gavilán, C., Margolles, A. (2013). Antibiotic resistance in probiotic bacteria. Front Microbiol.18(4): 202. doi: 10.3389/fmicb.2013.00202. eCollection 2013.

    Hatakka, K., Savilahti, E., Ponka, A., Meurman, J. H., Poussa, T., Naese, L. et al. (2001). Effect of long term consumption of probiotic milk on infections in children attending day care centres: double blind, randomised trial. BMJ. 2: 1318-1319.

    Havenarr, R., (1992). Selection of strains for probiotic use. In: R. Fuller (Eds.). Probiotics: The Scientific Basis. London, Chapman and Hall: 209-224.

    Hong, H.A., Duc le, H., and Cutting, S.M. (2005). The use of bacterial spore formers as probiotics. FEMS Microbiol Rev. 29: 813-835.

    Hilton, E., Isenberg, H. D., Alperstein, P., France, K., Borenstein, M.T. (1992). Ingestion of yogurt containing Lactobacillus acidophilus as prophylaxis for candidal vaginitis. Ann Intern Med.116: 353-357.

    Jacobsen, C. N., Rosenfeldt Nielsen, V., Hayford, A. E., Møller, P. L., Michaelsen, K. F., Paerregaard, A., Sandstrom, B., Tvede, M., Jakobsen, M. (1999). Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl. Environ. Microbiol. 65: 4949-4956.

    Jensen, H., Grimmer, S., Naterstad, K., Axelsson, L. (2012). In vitro testing of commercial and potential probiotic lactic acid bacteria. Int J Food Microbiol. 153: 216-222.

    Kolader, M. E., Vinh, H., Ngoc Tuyet, P. T., Thompson, C., Wolbers, M., Merson, L., Campbell, J.I., Ngoc Dung, T.T., Manh Tuan, H., Vinh Chau, N.V., Farrar, J., van Doorn, H. R., Baker, S. (2013). An oral preparation of Lactobacillus acidophilus for the treatment of uncomplicated acute watery diarrhoea in Vietnamese children: study protocol for a multicentre, randomised, placebo-controlled trial. Trials. 28 (14): 27. doi: 10.1186/1745-6215-14-27.

    McDonough, F. E., Hitchins, A. D., Wong, N. P., Wells, P., Bodwell, C. E. (1987). Modification of sweet acidophilus milk to improve utilization by lactose-intolerant persons. Am J Clin Nutr. 45: 570-574.

    Meurman, JH. (2005). Probiotics: do they have a role in oral medicine and dentistry? Eur J Oral Sci. 113: 188-196.

    Nimrat, S. and Vuthiphandchai, V. (2011). In vitro evaluation of commercial probiotic products used for marine shrimp cultivation in Thailand. African Journal of Biotechnology. 10(22): 4643-4650.

    Perdigon, G., Alvarez, S., Rachid, M., Aguero, G., Gobbato, N (1995). Immune system stimulation by probiotics. J Dairy Sci. 35: 412-420.

    Simpson, P. J., Fitzgerald, G. F., Stanton, C. and Ross, R. P. (2004). The evaluation of a mupirocin-based selective medium for the enumeration of bifidobacteria from probiotic animal feed. J. Microbiol. Methods. 57: 9-16.

    Tagg, J. R., Dajani, A. S., Wannamaker, L. W. (1976). Bacteriocins of Gram-positive bacteria. Bacteriol. Rev. 40: 722-756.

    Vanderhoof, J. A., Whitney, D. B., Antonson, D. L., Hanner, T. L., Lupo, J. V., Young, R. J. (1999). Lactobacillus GG in the prevention of antibiotic-associated diarrhea in children. J Pediatr. 135: 564-568.

    Von Bueltzingsloewen, I., Adlerberth, I., Wold, A. E., Dahlén, G., Jontell, M. (2003). Oral and intestinal microflora in 5-fluorouracil treated rats, translocation to cervical and mesenteric lymph nodes and effects of probiotic bacteria. Oral Microbiol Immunol. 18: 278-284.