Received: 25-07-2014
Accepted: 09-10-2015
DOI:
Views
Downloads
How to Cite:
In vitroGrowth and Development of Chrysanthemum sp. on the Iron Nano Supplemented Media
,
Nguyen Viet Cuong
2
,
Hoang Thanh Tung
2
,
Nguyen Thi Thanh Hien
2
,
Do Manh Cuong
2
,
Vu Thi Hien
2
,
Nguyen Ba Nam
2
,
Nguyen Phuc Huy
2
,
Vu Quoc Luan
2
,
Nguyen Hoai Chau
3
,
Ngo Quoc Buu
3
Keywords
Chrysanthemumsp., Fe-EDTA, growth and development, iron nano, chlorophyll
Abstract
The novel properties of nano metals including iron nano havemadethem a source of new materials which areapplied in a various fields inindustry, agriculture and medicine. However, the effects of iron nano on plant tissue culture have hardly been investigated. This study was conducted to determine the effects of individual iron nano with concentrations from 0 - 35 mg/l or in combination with Fe-EDTA on the growth and development of Chrysanthemumsp. cultured in vitro. In vitro Chrysanthemumshoots (2 cm high,2 pairs of leaves) and Chrysanthemumstem segments (2 node/segment) were used as the source materials for this experiment. After 30 days of culture, all shoots from stem segments on iron nano media (0 - 15 mg/l) appeared interveinal chlorosis with chlorophyll content of leaves significantly lower (from 8.433 to 24.667 μg/cm2) in comparison to shoot-inducing MS media shoots (39.567 μg/cm2). The combination of iron nano and Fe-EDTA, in contrast, showed better results. Shoots developedwell withoutinterveinal chlorosis. After 1 month of culture, all parameters of shoots on media supplemented with 15 mg/l iron nano were highest. On the other hand, the replacement of Fe-EDTA with iron nano affected the root morphology but did not promote the growth of Chrysanthemum(in comparison to the control). Roots in these medium were small and had less root hair than the roots of Chrysanthemumplanletson iron nano combined with Fe-EDTA media. 10 mg/l iron nano and 35 mg/l Fe-EDTA appeared as the best combination for the growth of Chrysanthemumplantlets.
References
Duncan D.B. (1995). Multiple range and multiple F test. Biometrics, 11: 1 - 42.
Eskandari H. (2011). The importance of iron (Fe) in plant products and mechanism of its uptake by plants. J. Appl. Environ. Biol. Sci., 1(10): 448 - 452.
Giordani T., Fabrizi A., Guidi L., Natali L., Giunti G., Ravasi F., Cavallini A. and Pardossi A. (2012). Response of tomato plants exposed to treatment with nanoparticles. EQA, 8: 27 - 38.
Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol., 15: 473 - 497.
Racuciu M. and Creanga D. (2006). TMA-OH coated magnetic nanoparticles internalize in vegetal tissue. Romanian J. Phys., 52: 395 - 402.
Seif S.M., Sorooshzadeh A.H., Rezazadeh S. and Naghdibadi H.A. (2011). Effect of nano-silver and silver nitrate on seed yield of borage. J. Med. Plant Res., 5(2): 171 - 175.
Slater A., Scott N.W. and Fowler M.R. (2008). Plant biotechnology: the genetic manipulation of plants. Chaper 2: Plant tissue culture. Oxford University Press, p. 41.
Trujillo-Reyesa J., Majumdara S., Botezc C.E., Peralta-Videaa J.R. and Gardea-Torresdeya J.L. (2014). Exposure studies of core-shell Fe/Fe3O4 and Cu/CuO NPs to lettuce (Lactuca sativa) plants: Are they a potential physiological and nutritional hazard?.J. Hazard. Mater.,267: 255 - 263.
Zhang W.Y. (2003). Nanoscale iron particles for environmental remediation: An overview. J. Nanopart. Res., 5: 323.
Zhu H., Han J., Xiao J.Q. and Jin Y. (2008). Uptake, translocation and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. J. Environ. Monit.,10: 713 - 717.