Received: 26-04-2016
Accepted: 22-10-2016
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Evaluation the Quality and Microbial Flora of King Grass Silage
,
Bui Quang Tuan
1
Keywords
King grass (Pennisetum purpureum), silage, microbial flora, lactic acid bacteria
Abstract
The aim of this study was to evaluate the quality and the microbial population of king grass (Pennisetum purpureophoides) silage. The experiment was carried out in a completely randomized design consisting of four treatments with three replications, namely control: traditional king grass silage without additive as a control; CT1: king grass silage with 5% salt; CT2: king grass silage with 5% molasses and CT4: king grass silage with 5% salt and 5% molasses. All silages were prepared using plastic jars (5kg) and incubated at room temperature (25 - 30oC) for 90 days. Silages were analyzed for fermentation characteristics and microbial diversity. The result confirmed that king grass ensilaged with molasses (CT2, CT3) had good fermentation characteristics compared with control and CT1 group. The silage (CT2 & CT3) showed golden yellow color, pleasant and acidic smell, no viscosity, and no mold growth with pH value below 4,2 (3.98 - 4.18). DM and crude protein were reduced by 1.15 % and 0.35% respectively. The highest content of lactic acid (1.30 - 1.58%) and the lowest ratio of ammonia nitrogen to the total nitrogen (5.11 -7.03%) were observed in CT2 and CT3. The microbial diversity in silages indicated that there was a highest population of lactic acid bacteria in CT2 and CT3 (194.17x106 - 316.33x106 CFU/g). The number of Bacillus sp. and Clostridium sp. mold and yeast were highest in control and CT1 (P < 0,05).
References
Bùi Xuân An (1998). Sử dụng hợp lý dây đậu phộng làm thức ăn cho gia súc nhai lại trên vùng miền Đông Nam Bộ. Luận án Tiến sỹ, Đại học Nông Lâm, Thành phố Hồ Chí Minh.
Atlas R.M. (1993). Handbook of Microbiological Media, Ed. by Parks L., CRC Press, Inc.
Đinh Văn Mười (2012). Nghiên cứu xác định tỷ lệ tiêu hoá, giá trị dinh dưỡng và xây dựng phương trình chẩn đoán các giá trị này của một số loại thức ăn dùng cho gia súc nhai lại, Luận án tiến sĩ nông nghiệp, Viện Chăn nuôi.
Bùi Quang Tuấn, Vũ Duy Giảng, Nguyễn Trọng Tiến, Nguyễn Xuân Trạch, Tôn Thất Sơn (2006). Ủ chua thân cây ngô sau thu bắp làm thức ăn cho bò sữa tại Đan Phượng, Hà Tây cũ. Tạp chí Chăn nuôi, 2: 18 - 21.
De Man J.D., Rogosa M., Sharpe M.E. (1960). A Medium for the Cultivation of Lactobacilli". J Appl Bact., 23: 130 - 135.
Đoàn Đức Vũ, Đặng Phước Chung và Nguyễn Thị Hiệp (2008). Nghiên cứu kỹ thuật ủ chua thân đậu phộng (lạc) làm thức ăn cho bò sữa, bò thịt. Tạp chí Chăn nuôi, 6: 21 - 25.
Hristov, A. N., T. A. McAllister and K. J. Cheng (2000). Intraruminal supplementation with increasing rates of exogenous polysaccharide degrading enzymes: Effects on nutrient digestion in cattle fed a barley grain diet. J. Anim. Sci., 78: 477 - 487.
Kung L. and R. Shaver (2001). Interpretation and use of silage fermentation analysis reports. Focus on Forage, University of Wisconsin Extension, 3(13): 1 - 5.
Muck R. E. and J. T. Dickerson (1988). Storage temperature effects on proteolysis in alfalfa silage. Transactions of the ASAE, 31(4): 1005 - 1009.
Piltz J. W. and Kaiser A. G. (2006). Principles of silage preservation. In: Successful silage (TopFodder silage manual). NSW Department of Primary Industries, pp. 25 - 56.