Received: 23-03-2016
Accepted: 20-09-2016
DOI:
Views
Downloads
How to Cite:
Application of RT-PCRfor Detection of InfectiousbronchitisDisease in Laying Hens in Some Northern Provinces of Vietnam
,
Nguyen ThiLoan
2, 3
,
Le Dinh Quyen
4
,
Duong Hong Quan
5
,
Le Huynh Thanh Phuong
2
,
Nguyen Ba Hien
2
Keywords
Chicken, infectious bronchitis, infectious bronchitis virus, RT-PCR, Vietnam
Abstract
Infectious bronchitis (IB) is one of the severe infectious disease causing tremendous economic losses in the poultry industry. In order to have an effective strategy for disease prevention and control, a method for rapid and accurate dectection of infectious bronchitis virus (IBV) is urgently needed. In this study, the tissue samples oflung, trachea and kidney collected from IB-suspected laying hens in three hen farms in Hanoi (Ba Vi District), Thai Nguyen and Bac Ninh have been diagnosed by the RT-PCR method. The diagnostic results showed that 34/71 (47,88%) samples were positive with the IBV.The common clinical signs ofIBV-infected hens included fever, depression, loss ofappetite,dyspnea, coughing, sneezing, tracheal rales, nasal discharge, diarrhea, fluid retention in the abdomen, facial swelling and conjunctivitis. The pathological signs in IBV-infected hensincluded kidney swelling (90%), tracheal hemorrhagea (77.5%), pulmonary hemorrhage and congestion(63,7%), inflammation of airsacculitis (60%) and upper respiratory tract (57,5%). Egg production and quality in IBV-infected laying hensdecreased by 20,7to 43%.
References
Ahmed Z., Naeem K. and Hameed A. (2007). Detection and seroprevalence of infectious bronchitis virus strains in commercial poultry in Pakistan. Poult Sci., 86: 1329 - 1335.
Al - Shekaili T., Baylis M. and Ganapathy K. (2015). Molecular detection of infectious bronchitis and avian metapneumoviruses in Oman backyard poultry. Res Vet Sci., 99: 46 - 52.
Cavanagh D. (2005). Coronaviruses in poultry and other birds. Avian Pathol., 34: 439 - 448.
Dolz R., Vergara - Alert J., Perez M., Pujols J. and Majo N. (2012). New insights on infectious bronchitis virus pathogenesis: characterization of Italy 02 serotype in chicks and adult hens. Vet Microbiol., 156: 256 - 264.
Feng J., Hu Y., Ma Z., Yu Q., Zhao J., Liu X. and Zhang G. (2012). Virulent avian infectious bronchitis virus, People's Republic of China. Emerg Infect Dis., 18: 1994 - 2001.
Huỳnh Thị Mỹ Lệ và Lê Văn Phan(2014). Một số biểu hiện lâm sàng và kết quả chẩn đoán ổ dịch ở đàn vịt trời. Tạp chí KHKT thú y, XXI(5): 59 - 67.
Ignjatovic J. and Sapats S. (2000). Avian infectious bronchitis virus. Rev Sci Tech., 19: 493 - 508.
Jahantigh M., Salari S. and Hedayati M. (2013). Detection of infectious bronchitis virus serotypes by reverse transcription polymerase chain reaction in broiler chickens. Springerplus, 2: 36.
Jones R.M., Ellis R.J., Cox W.J., Errington J., Fuller C., Irvine R.M. and Wakeley P.R. (2011). Development and validation of RT - PCR tests for the detection and S1 genotyping of infectious bronchitis virus and other closely related gamma coronaviruses within clinical samples. Transbound Emerg Dis., 58: 411 - 420.
Nguyễn Thị Phước Ninh (2008). Bài giảng bệnh truyền nhiễm chung trên gia cầm. Tủ sách Trường đại họcNông lâm thành phố Hồ Chí Minh.
OIE.(2012). Manual of standards for Diagnostic Tests and Vaccines. Chapter 2.3.2. Avian infectious bronchitis.
Rashid S., Naeem K., Ahmed Z., Saddique N., Abbas M.A. and Malik S.A. (2009). Multiplex polymerase chain reaction for the detection and differentiation of avian influenza viruses and other poultry respiratory pathogens. Poult Sci., 88: 2526 - 2531.
Saba Shirvan A. and Mardani K. (2014). Molecular detection of infectious bronchitis and Newcastle disease viruses in broiler chickens with respiratory signs using Duplex RT - PCR. Vet Res Forum, 5: 319 - 23.
Võ Thị Trà An, Nguyễn Thị Kim Yến, Hồ Hoàng Dũng(2012). Phân lập xác định serotype virusviêm phế quản truyền nhiễm từ gà thịt. Tạp chí KHKT thú y, XIX(3): 5-9.