Received: 18-04-2017
Accepted: 26-07-2017
DOI:
Views
Downloads
How to Cite:
Fast Freezing Cryopreservation of Dong Tao Chicken Sperms
Keywords
Artificial insemination, Dong Tao chicken breed, sperm cryopreservation
Abstract
A study on sperm cryopreservation and artificial insemination of Dong Tao chicken breed was conducted. Three experiments were carried out (1) Assessement of semen parameters of Dong Tao cocks in hot and cold seasons; (2) Comparison of efficiency ofcryopreservationof Dong Tao chicken sperm using rice straws (0.25 and 1.00 ml) and pellets; and (3) Evaluation of insemination efficiency of Dong Tao hens by cryopreserved sperms. SixDong Tao cocks and 48 Dong Tao hens (10 - 14 months old) were usedin this experiment. The results showed that Dong Tao cock’s semen quality wasgood and better in hot season compared to cold season. Sperm cryopreservation in cryo - straw forms wasbetter than in cryovial form. Thawed sperm quality parameters of 1 ml cryo - straw sperm samples were better than in 0.25 ml cryo - straw sperm samples. Artificial insemination with thawed sperms from1 ml cryo - strawsamples yielded highest percentage of fertilized eggs(70%),equal tonatural insemination.
References
Abu Md. Mamun Tarif, Mohammad Musharraf Uddin Bhuiyan, Raihana Nasrin Ferdousy, Nasrin Sultana Juyena and Md. Bazlur Rahman Mollah (2013). Evaluation of semen quality among four chicken lines, IOSR Journal of Agriculture and Veterinary Science (IOSR - JAVS), e - ISSN: 2319 - 2380, p - ISSN: 2319 - 2372, 6(5): 7-13.
Amann RP, Gill S. P. S and Hammerstedt R. H. (1997). Maximizing Genetic Impact of Roosters: Effective Handling, Extension and Utilization of Rooster Semen and Artificial Insemination, BioPore, Inc. State College, PA, 14(6): 1-15.
Ehling C., Taylor U., Baulain U., Weigend S., Henning M., and Rath D. (2012). Cryopreservation of semen from genetic resource chicken lines, Agriculture and Forestry Research, 62: 151-158.
Giesen A. F and Sexton T. J. (1983). Beltsville poultry semen extender, 7. Comparison of commercial diluents for holding turkey semen six hours at 150C, PMID: 6835912. Poult Sci., 62(2): 379-381.
Hanzawa S., Niinomi T., Miyata T., Tsutsui M and Tajima A. (2010). Cryopreservation of chicken semen using glycerol and N - methylacetamide as cryoprotective agent, Jap J Poult Sci., 47: J27-J32.
Johnson L. A., Weitze K. F., Fiser P. and Maxwell W. M. C. (2000). Storage of boar semen. Animal Reproduction Science, 62: 143-172.
Lê Thị Thu Hiền, Phùng Đức Tiến, Nguyễn Quý Khiêm và Nguyễn Thị Tình (2014). Bảo tồn và khai thác nguồn gen gà Đông Tảo. Tài liệu số hóa của Viện chăn nuôi. Truy cập tại: http://vcn. vnn. vn/uploads/files/B%E1%BA%A3o%20t%E1%BB%93n%20ngu%E1%BB%93n%20gen/Bao%20ton%20%20phat%20trien/B%E1%BA%A2O%20T %E1%BB%92N%20V%C3%80%20KHAI%20TH%C3%81C%20NGU%E1%BB%92N%20GEN%20G%C3%80%20%C4%90%C3%94NG%20T%E1%BA%A2O. pdf).
Ngo T. T. (2010). A Simplified responsiveness test for testing storage effects on liquid preserved boar spermatozoa, Master thesis, Hannover University of Veterinary Medicine, Germany.
Phillips J. J, Bramwell R. K and Graham J. K. (1996). Cryopreservation of rooster sperm using methyl cellulose, Poult. Sci., 75: 915-923.
Đào Đức Thà (2006). Kỹ thuật thụ tinh nhân tạo vật nuôi. Nhà xuất bản Lao động và Xã hội.
Nguyễn Đăng Vang, Trần Công Xuân, Phùng Đức Tiến, Lê Thị Nga và Nguyễn Mạnh Hùng (1999). Khả năng sản xuất của gà Đông Tảo nuôi tại Thuỵ Phương, Chuyên san Chăn nuôi gia cầm, Hội Chăn nuôi Việt Nam, tr. 114-116.
Wilson H. R. (1991). Physiological requirements of the developing embryo: Temperature and turning. In: Tullett S. G., Avian Incubation. Butterworth - Heinemann, London, UK, pp. 145-156.